PMID- 20943203
OWN - NLM
STAT- MEDLINE
DCOM- 20110429
LR  - 20211203
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 332
IP  - 1-2
DP  - 2011 Jan 30
TI  - Estrogen regulation of human with-no-lysine (K) kinase-4 gene expression involves 
      AP-1 transcription factor.
PG  - 140-8
LID - 10.1016/j.mce.2010.10.006 [doi]
AB  - With-no-lysine (K) kinase-4 (WNK4) is a serine/threonine protein kinase and plays 
      a crucial role in the regulation of blood pressure and electrolyte homeostasis. 
      Whether or not estrogen, an important regulator of physiologic functions, 
      modulates human WNK4 (hWNK4) gene expression is unknown. In the current study, 
      real-time PCR assay showed that 17beta-estradiol (E(2)) with estrogen receptor alpha 
      (ERalpha) suppressed the level of hWNK4 mRNA in cultured human embryo kidney 293 
      cells (HEK293). Luciferase activity analysis of the truncated hWNK4 promoters 
      indicated that a regulatory region from -216 to -202 is essential for the basal 
      transcriptional activity and response to E(2)/ERalpha. Using an electrophoresis 
      mobility shift assay and a chromatin immunoprecipitation assay, we identified an 
      activator protein-1 (AP-1) element at position -215/-205, to which AP-1 binding 
      was enhanced by E(2)/ERalpha. Accordingly, AP-1 protein was increased by E(2)/ERalpha 
      using Western blot analysis. Moreover, re-chromatin-immunoprecipitation and 
      co-immunoprecipitation assays showed a direct interaction between ERalpha and AP-1 in 
      HEK293 cells. In summary, these data document that E(2)/ERalpha regulates hWNK4 
      expression partly through AP-1 binding to the hWNK4 promoter.
CI  - Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Zhang, Yuanyuan
AU  - Zhang Y
AD  - Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 
      China.
FAU - Li, Chunyi
AU  - Li C
FAU - Li, Wei
AU  - Li W
FAU - Zhao, Yanyan
AU  - Zhao Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101011
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Transcription Factor AP-1)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (WNK4 protein, human)
SB  - IM
MH  - Base Sequence
MH  - Estradiol/*metabolism
MH  - Estrogen Receptor alpha/genetics/metabolism
MH  - *Gene Expression
MH  - HEK293 Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic
MH  - Protein Serine-Threonine Kinases/*genetics/metabolism
MH  - Transcription Factor AP-1/genetics/*metabolism
MH  - Transcription, Genetic
EDAT- 2010/10/15 06:00
MHDA- 2011/04/30 06:00
CRDT- 2010/10/15 06:00
PHST- 2010/06/29 00:00 [received]
PHST- 2010/09/14 00:00 [revised]
PHST- 2010/10/05 00:00 [accepted]
PHST- 2010/10/15 06:00 [entrez]
PHST- 2010/10/15 06:00 [pubmed]
PHST- 2011/04/30 06:00 [medline]
AID - S0303-7207(10)00494-6 [pii]
AID - 10.1016/j.mce.2010.10.006 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2011 Jan 30;332(1-2):140-8. doi: 10.1016/j.mce.2010.10.006. 
      Epub 2010 Oct 11.