PMID- 20943524
OWN - NLM
STAT- MEDLINE
DCOM- 20110405
LR  - 20220408
IS  - 0027-5514 (Print)
IS  - 0027-5514 (Linking)
VI  - 103
IP  - 1
DP  - 2011 Jan-Feb
TI  - Prevalence of an intraspecific Neotyphodium hybrid in natural populations of 
      stout wood reed (Cinna arundinacea L.) from eastern North America.
PG  - 75-84
LID - 10.3852/10-154 [doi]
AB  - Members of genus Neotyphodium are asexual derivatives of sexual Epichloe species 
      and maintain endophytic relationships with many cool-season grasses. Most 
      Neotyphodium species analyzed so far are interspecific hybrids with combined or 
      partial genomes of two or three ancestral species. In this study we characterized 
      Neotyphodium isolates from Cinna arundinacea, a perennial cool-season grass from 
      eastern North America. A total of 23 isolates grouping into two distinct 
      morphotypes were obtained from five local populations of C. arundinacea. PCR 
      amplification and cloning of translation-elongation factor 1-alpha (tefA) and 
      beta-tubulin (tubB) genes of 10 isolates comprising both morphotypes (two isolates 
      per location) revealed that all 10 contain two copies of tefA and tubB genes. 
      Surprisingly phylogenetic analysis of mainly non-coding sequence from these genes 
      revealed that both copies in each isolate were inherited from Epichloe typhina 
      ancestors, indicating that the C. arundinacea endophytes arose through 
      intraspecific hybridization between two E. typhina progenitors with extant 
      relatives infecting hosts Poa nemoralis and Poa pratensis. Furthermore the tefA 
      sequences were identical between isolates, as were tubB sequences, despite 
      obvious morphological differences. Profiling of alkaloid biosynthetic genes from 
      these isolates indicated the presence of the peramine biosynthetic gene (perA) 
      and the absence of genes required for biosynthesis of lolines, indole-diterpenes 
      and ergot alkaloids. Thus this endophyte is potentially capable of producing 
      peramine in planta and providing protection to its host from insect pests. The 
      absence of genes for indole-diterpenes and ergot alkaloid biosynthesis makes this 
      endophyte a candidate for agricultural applications. Based on our phylogenetic 
      analysis, alkaloid profiling and description of morphological characteristics, we 
      propose the name Neotyphodium schardlii for these isolates from C. arundinacea, a 
      new member of genus Neotyphodium and the first described to have arisen through 
      intraspecific hybridization.
FAU - Ghimire, Sita R
AU  - Ghimire SR
AD  - Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73401, 
      USA.
FAU - Rudgers, Jennifer A
AU  - Rudgers JA
FAU - Charlton, Nikki D
AU  - Charlton ND
FAU - Young, Carolyn
AU  - Young C
FAU - Craven, Kelly D
AU  - Craven KD
LA  - eng
SI  - GENBANK/HM138504
SI  - GENBANK/HM138505
SI  - GENBANK/HM138506
SI  - GENBANK/HM138507
PT  - Journal Article
DEP - 20100831
PL  - England
TA  - Mycologia
JT  - Mycologia
JID - 0400764
RN  - 0 (DNA, Fungal)
RN  - 0 (Peptide Elongation Factor 1)
RN  - 0 (Tubulin)
SB  - IM
MH  - Base Sequence
MH  - DNA, Fungal/chemistry/genetics
MH  - Hybridization, Genetic
MH  - Indiana
MH  - Molecular Sequence Data
MH  - Neotyphodium/genetics/*isolation & purification/ultrastructure
MH  - Peptide Elongation Factor 1/chemistry/genetics
MH  - Phylogeny
MH  - Poaceae/*microbiology
MH  - Polymerase Chain Reaction
MH  - Sequence Alignment
MH  - Symbiosis
MH  - Tubulin/chemistry/genetics
EDAT- 2010/10/15 06:00
MHDA- 2011/04/06 06:00
CRDT- 2010/10/15 06:00
PHST- 2010/10/15 06:00 [entrez]
PHST- 2010/10/15 06:00 [pubmed]
PHST- 2011/04/06 06:00 [medline]
AID - 10-154 [pii]
AID - 10.3852/10-154 [doi]
PST - ppublish
SO  - Mycologia. 2011 Jan-Feb;103(1):75-84. doi: 10.3852/10-154. Epub 2010 Aug 31.