PMID- 20943792
OWN - NLM
STAT- MEDLINE
DCOM- 20101223
LR  - 20240109
IS  - 1938-3207 (Electronic)
IS  - 0002-9165 (Linking)
VI  - 92
IP  - 6
DP  - 2010 Dec
TI  - Quercetin is equally or more effective than resveratrol in attenuating tumor 
      necrosis factor-alpha-mediated inflammation and insulin resistance in primary 
      human adipocytes.
PG  - 1511-21
LID - 10.3945/ajcn.2010.29807 [doi]
AB  - BACKGROUND: Quercetin and trans-resveratrol (trans-RSV) are plant polyphenols 
      reported to reduce inflammation or insulin resistance associated with obesity. 
      Recently, we showed that grape powder extract, which contains quercetin and 
      trans-RSV, attenuates markers of inflammation in human adipocytes and macrophages 
      and insulin resistance in human adipocytes. However, we do not know how quercetin 
      and trans-RSV individually affected these outcomes. OBJECTIVE: The aim of this 
      study was to examine the extent to which quercetin and trans-RSV prevented 
      inflammation or insulin resistance in primary cultures of human adipocytes 
      treated with tumor necrosis factor-alpha (TNF-alpha)-an inflammatory cytokine elevated in 
      the plasma and adipose tissue of obese, diabetic individuals. DESIGN: Cultures of 
      human adipocytes were pretreated with quercetin and trans-RSV followed by 
      treatment with TNF-alpha. Subsequently, gene and protein markers of inflammation and 
      insulin resistance were measured. RESULTS: Quercetin, and to a lesser extent 
      trans-RSV, attenuated the TNF-alpha-induced expression of inflammatory genes such as 
      interleukin (IL)-6, IL-1beta, IL-8, and monocyte chemoattractant protein-1 (MCP-1) 
      and the secretion of IL-6, IL-8, and MCP-1. Quercetin attenuated TNF-alpha-mediated 
      phosphorylation of extracellular signal-related kinase and c-Jun-NH(2) terminal 
      kinase, whereas trans-RSV attenuated only c-Jun-NH(2) terminal kinase 
      phosphorylation. Quercetin and trans-RSV attenuated TNF-alpha-mediated 
      phosphorylation of c-Jun and degradation of inhibitory kappaB protein. Quercetin, but 
      not trans-RSV, decreased TNF-alpha-induced nuclear factor-kappaB transcriptional 
      activity. Quercetin and trans-RSV attenuated the TNF-alpha-mediated suppression of 
      peroxisome proliferator-activated receptor gamma (PPARgamma) and PPARgamma target genes and 
      of PPARgamma protein concentrations and transcriptional activity. Quercetin prevented 
      the TNF-alpha-mediated serine phosphorylation of insulin receptor substrate-1 and 
      protein tyrosine phosphatase-1B gene expression and the suppression of 
      insulin-stimulated glucose uptake, whereas trans-RSV prevented only the 
      TNF-alpha-mediated serine phosphorylation of insulin receptor substrate-1. 
      CONCLUSION: These data suggest that quercetin is equally or more effective than 
      trans-RSV in attenuating TNF-alpha-mediated inflammation and insulin resistance in 
      primary human adipocytes.
FAU - Chuang, Chia-Chi
AU  - Chuang CC
AD  - Department of Nutrition, University of North Carolina-Greensboro, Greensboro, NC, 
      USA.
FAU - Martinez, Kristina
AU  - Martinez K
FAU - Xie, Guoxiang
AU  - Xie G
FAU - Kennedy, Arion
AU  - Kennedy A
FAU - Bumrungpert, Akkarach
AU  - Bumrungpert A
FAU - Overman, Angel
AU  - Overman A
FAU - Jia, Wei
AU  - Jia W
FAU - McIntosh, Michael K
AU  - McIntosh MK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101013
PL  - United States
TA  - Am J Clin Nutr
JT  - The American journal of clinical nutrition
JID - 0376027
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Insulin)
RN  - 0 (PPAR gamma)
RN  - 0 (Plant Extracts)
RN  - 0 (Stilbenes)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9IKM0I5T1E (Quercetin)
RN  - IY9XDZ35W2 (Glucose)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Adipocytes/drug effects/metabolism
MH  - Adult
MH  - Anti-Inflammatory Agents/pharmacology/*therapeutic use
MH  - Female
MH  - Gene Expression
MH  - Glucose/metabolism
MH  - Humans
MH  - Inflammation/*drug therapy/genetics/metabolism
MH  - Insulin/metabolism
MH  - *Insulin Resistance
MH  - Middle Aged
MH  - PPAR gamma/metabolism
MH  - Phosphorylation
MH  - Phytotherapy
MH  - Plant Extracts/pharmacology/*therapeutic use
MH  - Quercetin/pharmacology/*therapeutic use
MH  - Resveratrol
MH  - Signal Transduction/drug effects
MH  - Stilbenes/pharmacology/*therapeutic use
MH  - Transcription, Genetic/drug effects
MH  - Tumor Necrosis Factor-alpha/*metabolism
MH  - Vitis/chemistry
MH  - Young Adult
EDAT- 2010/10/15 06:00
MHDA- 2010/12/25 06:00
CRDT- 2010/10/15 06:00
PHST- 2010/10/15 06:00 [entrez]
PHST- 2010/10/15 06:00 [pubmed]
PHST- 2010/12/25 06:00 [medline]
AID - S0002-9165(23)02160-3 [pii]
AID - 10.3945/ajcn.2010.29807 [doi]
PST - ppublish
SO  - Am J Clin Nutr. 2010 Dec;92(6):1511-21. doi: 10.3945/ajcn.2010.29807. Epub 2010 
      Oct 13.