PMID- 20945438
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20211020
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Print)
IS  - 0021-9541 (Linking)
VI  - 226
IP  - 5
DP  - 2011 May
TI  - Reprogramming the pluripotent cell cycle: restoration of an abbreviated G1 phase 
      in human induced pluripotent stem (iPS) cells.
PG  - 1149-56
LID - 10.1002/jcp.22440 [doi]
AB  - Induced pluripotent stem (iPS) cells derived from terminally differentiated human 
      fibroblasts are reprogrammed to possess stem cell like properties. However, the 
      extent to which iPS cells exhibit unique properties of the human embryonic stem 
      (hES) cell cycle remains to be established. hES cells are characterized by an 
      abbreviated G1 phase ( approximately  2.5 h) and accelerated organization of subnuclear domains 
      that mediate the assembly of regulatory machinery for histone gene expression 
      [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters 
      of iPS cells in comparison to hES cells. Analysis of DNA synthesis 
      [5-bromo-2'-deoxy-uridine (BrdU) incorporation], cell cycle distribution (FACS 
      analysis and Ki67 staining) and subnuclear organization of HLBs 
      [immunofluorescence microscopy and fluorescence in situ hybridization (FISH)] 
      revealed that human iPS cells have a short G1 phase ( approximately  2.5 h) and an abbreviated 
      cell cycle (16-18 h). Furthermore, HLBs are formed and reorganized rapidly after 
      mitosis (within 1.5-2 h). Thus, reprogrammed iPS cells have cell cycle kinetics 
      and dynamic subnuclear organization of regulatory machinery that are principal 
      properties of pluripotent hES cells. Our findings support the concept that the 
      abbreviated cell cycle of hES and iPS cells is functionally linked to 
      pluripotency.
CI  - Copyright (c) 2010 Wiley-Liss, Inc.
FAU - Ghule, Prachi N
AU  - Ghule PN
AD  - Department of Cell Biology and Cancer Center, University of Massachusetts Medical 
      School, Worcester, Massachusetts 01655, USA.
FAU - Medina, Ricardo
AU  - Medina R
FAU - Lengner, Christopher J
AU  - Lengner CJ
FAU - Mandeville, Matthew
AU  - Mandeville M
FAU - Qiao, Meng
AU  - Qiao M
FAU - Dominski, Zbigniew
AU  - Dominski Z
FAU - Lian, Jane B
AU  - Lian JB
FAU - Stein, Janet L
AU  - Stein JL
FAU - van Wijnen, Andre J
AU  - van Wijnen AJ
FAU - Stein, Gary S
AU  - Stein GS
LA  - eng
GR  - R01 CA139322/CA/NCI NIH HHS/United States
GR  - R01 CA082834/CA/NCI NIH HHS/United States
GR  - P01 CA082834-11/CA/NCI NIH HHS/United States
GR  - R37 DE012528/DE/NIDCR NIH HHS/United States
GR  - R01 CA139322-02/CA/NCI NIH HHS/United States
GR  - P01 CA082834/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cell Line
MH  - Cell Nucleus/metabolism/*physiology
MH  - DNA Replication
MH  - Embryonic Stem Cells/metabolism/*physiology
MH  - *G1 Phase/genetics
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Induced Pluripotent Stem Cells/metabolism/*physiology
MH  - Ki-67 Antigen/metabolism
MH  - Kinetics
MH  - Microscopy, Fluorescence
MH  - *Mitosis/genetics
MH  - Transcription Factors/genetics/metabolism
PMC - PMC3045197
MID - NIHMS271380
EDAT- 2010/10/15 06:00
MHDA- 2011/04/16 06:00
PMCR- 2012/05/01
CRDT- 2010/10/15 06:00
PHST- 2010/10/15 06:00 [entrez]
PHST- 2010/10/15 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - 10.1002/jcp.22440 [doi]
PST - ppublish
SO  - J Cell Physiol. 2011 May;226(5):1149-56. doi: 10.1002/jcp.22440.