PMID- 20946858
OWN - NLM
STAT- MEDLINE
DCOM- 20110126
LR  - 20101015
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 471
DP  - 2010
TI  - Reversible histidine phosphorylation in mammalian cells: a teeter-totter formed 
      by nucleoside diphosphate kinase and protein histidine phosphatase 1.
PG  - 379-402
LID - 10.1016/S0076-6879(10)71020-X [doi]
AB  - Regulation of protein phosphorylation by kinases and phosphatases is involved in 
      many signaling pathways in mammalian cells. In contrast to prokaryotes and lower 
      eukaryotes a role for the reversible phosphorylation of histidine residues is 
      just emerging. The beta subunit of heterotrimeric G proteins, the metabolic enzyme 
      adenosine 5'-triphosphate-citrate lyase (ACL), and the Ca2+-activated K+ channel 
      KCa3.1 have been identified as targets for nucleoside diphosphate kinase (NDPK) 
      acting as protein histidine kinase and the so far only identified mammalian 
      protein histidine phosphatase (PHPT-1). Herein, we describe the analysis of the 
      phosphorylation and dephosphorylation of histidine residues by NDPK and PHPT-1. 
      In addition, experimental protocols for studying the consequences of 
      heterotrimeric G protein activation via NDPK/Gbetagamma mediated phosphorelay, the 
      regulation of ACL activity and of KCa3.1 conductivity by histidine 
      phosphorylation will be presented.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Wieland, Thomas
AU  - Wieland T
AD  - Institut fur Experimentelle und Klinische Pharmakologie und Toxikologie, 
      Medizinische Fakultat Mannheim, Universitat Heidelberg, Mannheim, Germany.
FAU - Hippe, Hans-Jorg
AU  - Hippe HJ
FAU - Ludwig, Katrin
AU  - Ludwig K
FAU - Zhou, Xiao-Bo
AU  - Zhou XB
FAU - Korth, Michael
AU  - Korth M
FAU - Klumpp, Susanne
AU  - Klumpp S
LA  - eng
PT  - Journal Article
DEP - 20100301
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
RN  - EC 3.1.3.16 (Protein Phosphatase 1)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Nucleoside-Diphosphate Kinase/*metabolism
MH  - Phosphorylation
MH  - Protein Phosphatase 1/*metabolism
EDAT- 2010/10/16 06:00
MHDA- 2011/01/28 06:00
CRDT- 2010/10/16 06:00
PHST- 2010/10/16 06:00 [entrez]
PHST- 2010/10/16 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
AID - S0076-6879(10)71020-X [pii]
AID - 10.1016/S0076-6879(10)71020-X [doi]
PST - ppublish
SO  - Methods Enzymol. 2010;471:379-402. doi: 10.1016/S0076-6879(10)71020-X. Epub 2010 
      Mar 1.