PMID- 20946947
OWN - NLM
STAT- MEDLINE
DCOM- 20110517
LR  - 20191210
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 25
IP  - 1
DP  - 2011 Feb
TI  - Precision-Cut Liver Slices of Salmo salar as a tool to investigate the oxidative 
      impact of CYP1A-mediated PCB 126 and 3-methylcholanthrene metabolism.
PG  - 335-42
LID - 10.1016/j.tiv.2010.10.002 [doi]
AB  - Fish isolated cell systems have long been used to predict in vivo toxicity of 
      man-made chemicals. In present study, we tested the suitability of Precision-Cut 
      Liver Slices (PCLS) as an alternative to these models that allows the evaluation 
      of a global tissue response to toxicants, to investigate oxidative stress 
      response to cytochrome P450 1A (CYP1A) induction in fish liver. PCLS of Salmo 
      salar were exposed for 21 h to increasing doses of 3-methylcholanthrene (3-MC) 
      and Polychlorobiphenyl 126 (PCB 126). 3-MC (25 muM) strongly induced CYP1A 
      transcription. In dose-response analysis (25-100 muM), EROD activity was strongly 
      increased at intermediate 3-MC concentrations. We found the counter-intuitive 
      decline of EROD at the highest 3-MC doses to result from reversible competition 
      with ethoxyresorufin. No increases of H(2)O(2) production, antioxidant enzymes 
      activities or oxidative damage to lipids were found with 3-MC treatments. PCLS 
      subjected to PCB 126 (2-200 nM) showed increased contamination levels and a 
      parallel increased CYP1A mRNA synthesis and EROD activity. H(2)O(2) production 
      tended to increase but no oxidative damage to lipids was found. As antioxidant 
      enzymes activities declined at the highest PCB 126 dose, it is suggested that 
      longer incubation periods could be required to generate oxidative stress in PCLS.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Lemaire, Benjamin
AU  - Lemaire B
AD  - Institut des Sciences de la Vie, Universite catholique de Louvain, 
      Louvain-la-Neuve, Belgium.
FAU - Beck, Michael
AU  - Beck M
FAU - Jaspart, Melanie
AU  - Jaspart M
FAU - Debier, Cathy
AU  - Debier C
FAU - Calderon, Pedro Buc
AU  - Calderon PB
FAU - Thome, Jean-Pierre
AU  - Thome JP
FAU - Rees, Jean-Francois
AU  - Rees JF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20101012
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (RNA, Messenger)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
RN  - 0 (Water Pollutants, Chemical)
RN  - 56-49-5 (Methylcholanthrene)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - TSH69IA9XF (3,4,5,3',4'-pentachlorobiphenyl)
SB  - IM
MH  - Animals
MH  - Citrate (si)-Synthase/metabolism
MH  - Cytochrome P-450 CYP1A1/*biosynthesis/genetics/metabolism
MH  - Enzyme Induction/drug effects
MH  - Hydrogen Peroxide/metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Liver/chemistry/*drug effects/metabolism
MH  - Methylcholanthrene/*toxicity
MH  - Organ Culture Techniques
MH  - Osmolar Concentration
MH  - Oxidative Stress/*drug effects
MH  - Oxidoreductases/metabolism
MH  - Polychlorinated Biphenyls/analysis/*toxicity
MH  - RNA, Messenger/metabolism
MH  - Salmo salar
MH  - Thiobarbituric Acid Reactive Substances/metabolism
MH  - Time Factors
MH  - Toxicity Tests
MH  - Water Pollutants, Chemical/*toxicity
EDAT- 2010/10/16 06:00
MHDA- 2011/05/18 06:00
CRDT- 2010/10/16 06:00
PHST- 2010/07/20 00:00 [received]
PHST- 2010/09/30 00:00 [revised]
PHST- 2010/10/04 00:00 [accepted]
PHST- 2010/10/16 06:00 [entrez]
PHST- 2010/10/16 06:00 [pubmed]
PHST- 2011/05/18 06:00 [medline]
AID - S0887-2333(10)00260-2 [pii]
AID - 10.1016/j.tiv.2010.10.002 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2011 Feb;25(1):335-42. doi: 10.1016/j.tiv.2010.10.002. Epub 
      2010 Oct 12.