PMID- 20947660
OWN - NLM
STAT- MEDLINE
DCOM- 20111207
LR  - 20240503
IS  - 1460-2083 (Electronic)
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 20
IP  - 2
DP  - 2011 Jan 15
TI  - Dystrophin conferral using human endothelium expressing HLA-E in the 
      non-immunosuppressive murine model of Duchenne muscular dystrophy.
PG  - 235-44
LID - 10.1093/hmg/ddq458 [doi]
AB  - Human leukocyte antigen (HLA)-E is a non-classical major histocompatibility 
      complex class I (Ib) molecule, which plays an important role in 
      immunosuppression. In this study, we investigated the immunomodulating effect of 
      HLA-E in a xenogeneic system, using human placental artery-derived endothelial 
      (hPAE) cells expressing HLA-E in a mouse model. In vitro cell lysis analysis by 
      primed lymphocytes in combination with siRNA transfection showed that HLA-E is 
      necessary for inhibition of the immune response. Similarly, in vivo cell 
      implantation analysis with siRNA-mediated down-regulation of HLA-E demonstrates 
      that HLA-E is involved in immunosuppression. As hPAE cells efficiently 
      transdifferentiate into myoblasts/myocytes in vitro, we transplanted the cells 
      into mdx mice, a model of Duchenne muscular dystrophy. hPAE cells conferred 
      dystrophin to myocytes of the 'immunocompetent' mdx mice with extremely high 
      efficiency. These findings suggest that HLA-E-expressing cells with a myogenic 
      potential represent a promising source for cell-based therapy of patients with 
      muscular dystrophy.
FAU - Cui, Chang-Hao
AU  - Cui CH
AD  - Department of Reproductive Biology, National Institute for Child Health and 
      Development, Tokyo, Japan.
FAU - Miyoshi, Shunichiro
AU  - Miyoshi S
FAU - Tsuji, Hiroko
AU  - Tsuji H
FAU - Makino, Hatsune
AU  - Makino H
FAU - Kanzaki, Seiichi
AU  - Kanzaki S
FAU - Kami, Daisuke
AU  - Kami D
FAU - Terai, Masanori
AU  - Terai M
FAU - Suzuki, Harumi
AU  - Suzuki H
FAU - Umezawa, Akihiro
AU  - Umezawa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101014
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (Dystrophin)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Arteries/cytology
MH  - Cell Culture Techniques
MH  - Disease Models, Animal
MH  - Dystrophin/*genetics/*metabolism
MH  - *Endothelial Cells/metabolism
MH  - Gene Expression Regulation/immunology
MH  - Histocompatibility Antigens Class I/*genetics/metabolism
MH  - Humans
MH  - Immunocompetence/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred mdx
MH  - Muscle Development/genetics
MH  - Muscular Dystrophy, Duchenne/*genetics/immunology/therapy
MH  - RNA, Small Interfering/genetics/metabolism
MH  - HLA-E Antigens
PMC - PMC3005899
EDAT- 2010/10/16 06:00
MHDA- 2011/12/13 00:00
PMCR- 2010/10/14
CRDT- 2010/10/16 06:00
PHST- 2010/10/16 06:00 [entrez]
PHST- 2010/10/16 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2010/10/14 00:00 [pmc-release]
AID - ddq458 [pii]
AID - 10.1093/hmg/ddq458 [doi]
PST - ppublish
SO  - Hum Mol Genet. 2011 Jan 15;20(2):235-44. doi: 10.1093/hmg/ddq458. Epub 2010 Oct 
      14.