PMID- 20950135
OWN - NLM
STAT- MEDLINE
DCOM- 20110105
LR  - 20101122
IS  - 1532-4303 (Electronic)
IS  - 0277-0903 (Linking)
VI  - 47
IP  - 10
DP  - 2010 Dec
TI  - Immunochromatographic assay for measurement of total IgE in tears, nasal mucus, 
      and saliva of patients with allergic rhinoconjunctivitis.
PG  - 1153-60
LID - 10.3109/02770903.2010.527025 [doi]
AB  - OBJECTIVE: Measurement of total immunoglobulin E (IgE) is clinically important 
      for the diagnosis of allergic diseases. The total serum IgE level is normally 
      measured because of its widespread use and convenience, but little attention has 
      been paid to the measurement of local IgE concentrations. We evaluated whether 
      the measurement of local production of IgE in tears, saliva, and nasal mucus was 
      useful for the diagnosis of allergic rhinoconjunctivitis. METHODS: A prospective, 
      nonrandomized, cross-sectional study was conducted in 33 consecutive patients 
      with seasonal allergic rhinoconjunctivitis (allergic group) and in 30 age- and 
      sex-matched healthy control subjects (control group). The total IgE level was 
      measured in tears, saliva, and nasal mucus from all subjects. Using a 4- or 
      5-point scale, symptoms (sneezing, rhinorrhea, nasal obstruction, ocular itching, 
      and lacrimation) were assessed in each subject along with the activities of daily 
      living (ADL) score and total symptom score for allergic conjunctivitis. RESULTS: 
      Total IgE could be assayed within 10 minutes of collection in all samples. The 
      scores for all symptoms were higher in the allergic group than in the control 
      group (p < .00001). The IgE scores for tear fluid samples (p < .00001) and 
      undiluted saliva (p = .00003) were significantly higher in the allergic group 
      than in the control group. The total IgE score of tear fluid samples was strongly 
      correlated with the severity of symptoms of allergic conjunctivitis, including 
      ocular itching (r = 0.769, p < .00001), tearing (r = 0.560, p = .00035), and 
      ocular symptom score (r = 0.329, p = .03452). On the contrary, the total IgE 
      scores for both saliva and nasal mucus were correlated with the severity of 
      rhinitis-related symptoms, including sneezing (saliva r = 0.897, p < .00001; 
      nasal mucus r = 0.871, p = .00024), nose blowing (saliva r = 0.764, p < .00001; 
      nasal mucus r = 0.829, p = .00080), and nasal obstruction (saliva r = 0.519, p = 
      .00099; nasal mucus r = 0.745, p = .00429). The ADL score was correlated with the 
      total IgE level in each specimen (tear r = 0.705, p < .00001; saliva r = 0.468, p 
      = .00301; nasal mucus r = 0.479, p = .06816). CONCLUSIONS: These results suggest 
      that local production of IgE is closely correlated with local allergic symptoms. 
      This rapid test for the measurement of local IgE is easy to perform on an 
      outpatient basis and may be helpful in the diagnosis of allergic rhinitis and 
      conjunctivitis.
FAU - Mimura, Tatsuya
AU  - Mimura T
AD  - Department of Ophthalmology, Toranomon Hospital, Minato-ku, Tokyo, Japan. 
      mimurat-tky@umin.ac.jp
FAU - Usui, Tomohiko
AU  - Usui T
FAU - Mori, Mikiro
AU  - Mori M
FAU - Aixinjueluo, Wei
AU  - Aixinjueluo W
FAU - Funatsu, Hideharu
AU  - Funatsu H
FAU - Noma, Hidetaka
AU  - Noma H
FAU - Amano, Shiro
AU  - Amano S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101101
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Conjunctivitis, Allergic/diagnosis/*immunology
MH  - Cross-Sectional Studies
MH  - Female
MH  - Humans
MH  - Immunoassay/*methods
MH  - Immunoglobulin E/*analysis
MH  - Male
MH  - Middle Aged
MH  - Mucus/*immunology
MH  - Predictive Value of Tests
MH  - Prospective Studies
MH  - Rhinitis, Allergic, Seasonal/diagnosis/*immunology
MH  - Saliva/*immunology
MH  - Sensitivity and Specificity
MH  - Statistics, Nonparametric
MH  - Tears/*immunology
MH  - Young Adult
EDAT- 2010/10/19 06:00
MHDA- 2011/01/06 06:00
CRDT- 2010/10/19 06:00
PHST- 2010/10/19 06:00 [entrez]
PHST- 2010/10/19 06:00 [pubmed]
PHST- 2011/01/06 06:00 [medline]
AID - 10.3109/02770903.2010.527025 [doi]
PST - ppublish
SO  - J Asthma. 2010 Dec;47(10):1153-60. doi: 10.3109/02770903.2010.527025. Epub 2010 
      Nov 1.