PMID- 20950251
OWN - NLM
STAT- MEDLINE
DCOM- 20110721
LR  - 20110228
IS  - 1937-3392 (Electronic)
IS  - 1937-3384 (Linking)
VI  - 17
IP  - 3
DP  - 2011 Mar
TI  - Expansion and hepatocytic differentiation of liver progenitor cells in vivo using 
      a vascularized tissue engineering chamber in mice.
PG  - 359-66
LID - 10.1089/ten.TEC.2009.0519 [doi]
AB  - Current cell-based treatment alternatives to organ transplantation for liver 
      failure remain unsatisfactory. Hepatocytes have a strong tendency to 
      dedifferentiate and apoptose when isolated and maintained in culture. In 
      contrast, liver progenitor cells (LPCs) are robust, easy to culture and have been 
      shown to replace damaged hepatocytes in liver disease. In this study we 
      investigate whether isolated LPCs can survive and differentiate toward mature 
      hepatocytes in vivo when implanted into a heterotopic mouse tissue engineering 
      chamber model. Healthy Balb/c mice and those put on a choline-deficient 
      ethionin-supplemented diet to induce chronic liver disease were implanted with a 
      tissue engineering chamber based on the epigastric flow through pedicle model, 
      containing either 1 x 10(6) LPCs suspended in Matrigel, or LPC-spheroids produced 
      by preculture for 1 week in Matrigel. Four weeks after implantation the chamber 
      contents were harvested. In all four groups, progenitor cells persisted in large 
      numbers to 4 weeks and demonstrated evidence of considerable proliferation judged 
      by Ki67-positive cells. Periodic acid Schiff staining demonstrated 
      differentiation of some cells into mature hepatocytes. Constructs grown from 
      LPC-spheroids demonstrated considerably greater LPC survival than those from LPCs 
      that were grown as monolayers and implanted as dissociated cells. The combined 
      use of LPC spheroids and the vascularized chamber model could be the basis for a 
      viable alternative to current treatments for chronic liver failure.
FAU - Forster, Natasha
AU  - Forster N
AD  - Bernard O'Brien Institute of Microsurgery, St. Vincent's Hospital, Fitzroy, 
      Australia.
FAU - Palmer, Jason A
AU  - Palmer JA
FAU - Yeoh, George
AU  - Yeoh G
FAU - Ong, Wei-Chen
AU  - Ong WC
FAU - Mitchell, Geraldine M
AU  - Mitchell GM
FAU - Slavin, John
AU  - Slavin J
FAU - Tirnitz-Parker, Janina
AU  - Tirnitz-Parker J
FAU - Morrison, Wayne A
AU  - Morrison WA
LA  - eng
PT  - Journal Article
DEP - 20101130
PL  - United States
TA  - Tissue Eng Part C Methods
JT  - Tissue engineering. Part C, Methods
JID - 101466663
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Animals
MH  - Blood Vessels/*metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Hepatocytes/*cytology
MH  - Ki-67 Antigen/metabolism
MH  - Liver/*cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Stem Cells/*cytology
MH  - Tissue Engineering/*instrumentation/*methods
MH  - Tissue Scaffolds
EDAT- 2010/10/19 06:00
MHDA- 2011/07/22 06:00
CRDT- 2010/10/19 06:00
PHST- 2010/10/19 06:00 [entrez]
PHST- 2010/10/19 06:00 [pubmed]
PHST- 2011/07/22 06:00 [medline]
AID - 10.1089/ten.TEC.2009.0519 [doi]
PST - ppublish
SO  - Tissue Eng Part C Methods. 2011 Mar;17(3):359-66. doi: 10.1089/ten.TEC.2009.0519. 
      Epub 2010 Nov 30.