PMID- 20951495 OWN - NLM STAT- MEDLINE DCOM- 20110310 LR - 20240109 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 185 IP - 1 DP - 2011 Jan 15 TI - Scale up of 2,4-dichlorophenol removal from aqueous solutions using Brassica napus hairy roots. PG - 269-74 LID - 10.1016/j.jhazmat.2010.09.028 [doi] AB - Chlorophenols are harmful pollutants, frequently found in the effluents of several industries. For this reason, many environmental friendly technologies are being explored for their removal from industrial wastewaters. The aim of the present work was to study the scale up of 2,4-dichlorophenol (2,4-DCP) removal from synthetic wastewater, using Brassica napus hairy roots and H(2)O(2) in a discontinuous stirred tank reactor. We have analyzed some operational conditions, because the scale up of such process was poorly studied. High removal efficiencies were obtained (98%) in a short time (30 min). When roots were re-used for six consecutive cycles, 2,4-DCP removal efficiency decreased from 98 to 86%, in the last cycle. After the removal process, the solutions obtained from the reactor were assessed for their toxicity using an acute test with Lactuca sativa L. seeds. Results suggested that the treated solution was less toxic than the parent solution, because neither inhibition of lettuce germination nor effects in root and hypocotyl lengths were observed. Therefore, we provide evidence that Brassica napus hairy roots could be effectively used to detoxify solutions containing 2,4-DCP and they have considerable potential for a large scale removal of this pollutant. Thus, this study could help to design a method for continuous and safe treatment of effluents containing chlorophenols. CI - Copyright (c) 2010 Elsevier B.V. All rights reserved. FAU - Angelini, Vanina A AU - Angelini VA AD - Departamento de Biologia Molecular, FCEFQN, Universidad Nacional de Rio Cuarto, 5800 Rio Cuarto, Cordoba, Argentina. FAU - Orejas, Joaquin AU - Orejas J FAU - Medina, Maria I AU - Medina MI FAU - Agostini, Elizabeth AU - Agostini E LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20100917 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 RN - 0 (Chlorophenols) RN - 0 (Pesticide Residues) RN - 0 (Soil Pollutants) RN - 0 (Solutions) RN - BBX060AN9V (Hydrogen Peroxide) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - Bioreactors MH - Brassica napus/*chemistry MH - Chlorophenols/analysis/*isolation & purification/toxicity MH - Hydrogen Peroxide/analysis MH - Lactuca/physiology MH - Oxidation-Reduction MH - Pesticide Residues/analysis/*isolation & purification/toxicity MH - Plant Roots/chemistry MH - Seeds MH - Soil Pollutants/analysis/*isolation & purification/toxicity MH - Solutions EDAT- 2010/10/19 06:00 MHDA- 2011/03/11 06:00 CRDT- 2010/10/19 06:00 PHST- 2010/05/26 00:00 [received] PHST- 2010/08/18 00:00 [revised] PHST- 2010/09/09 00:00 [accepted] PHST- 2010/10/19 06:00 [entrez] PHST- 2010/10/19 06:00 [pubmed] PHST- 2011/03/11 06:00 [medline] AID - S0304-3894(10)01187-8 [pii] AID - 10.1016/j.jhazmat.2010.09.028 [doi] PST - ppublish SO - J Hazard Mater. 2011 Jan 15;185(1):269-74. doi: 10.1016/j.jhazmat.2010.09.028. Epub 2010 Sep 17.