PMID- 20953058
OWN - NLM
STAT- MEDLINE
DCOM- 20110209
LR  - 20190827
IS  - 1348-2246 (Electronic)
IS  - 0910-6340 (Linking)
VI  - 26
IP  - 10
DP  - 2010
TI  - Simultaneous separation and determination of eleven nucleosides and bases in 
      beer, herring sperm DNA and RNA soft capsule by high-performance liquid 
      chromatography.
PG  - 1111-4
AB  - A simple and rapid high-performance liquid chromatography method was developed 
      for the determination of eleven nucleosides and bases in beer, herring sperm DNA 
      and RNA soft capsules. The separation was carried out on an Agilent extend-C(18) 
      column with a simple gradient elution of acetonitrile and water as the mobile 
      phase. Good linear relationships between the peak areas and the concentrations of 
      the analytes were obtained. The detection limits for eleven analytes were in the 
      range of 0.007-0.037 mg/L by UV detection at 260 nm. The relative standard 
      deviations (RSDs) of the retention times were in the range of 0.78-1.85% for 
      intra-day and 0.87-1.94% for inter-day, respectively. The RSDs of the peak areas 
      were in the range of 2.71-3.22% for intra-day and 3.03-3.39% for inter-day, 
      respectively. This method has been successfully applied to simultaneous 
      determination of eleven nucleosides and bases in beer, herring sperm DNA and RNA 
      soft capsules with the recoveries in the range of 93.7-108.3%.
FAU - Hou, Shengjie
AU  - Hou S
AD  - Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of 
      Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. 
      China.
FAU - Ding, Mingyu
AU  - Ding M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Anal Sci
JT  - Analytical sciences : the international journal of the Japan Society for 
      Analytical Chemistry
JID - 8511078
RN  - 0 (Nucleosides)
RN  - 0 (Purines)
RN  - 0 (Pyrimidines)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Beer/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - DNA/*chemistry
MH  - Fishes
MH  - Male
MH  - Nucleosides/*analysis
MH  - Purines/*analysis
MH  - Pyrimidines/*analysis
MH  - RNA/*chemistry
MH  - Spermatozoa/chemistry
EDAT- 2010/10/19 06:00
MHDA- 2011/02/10 06:00
CRDT- 2010/10/19 06:00
PHST- 2010/10/19 06:00 [entrez]
PHST- 2010/10/19 06:00 [pubmed]
PHST- 2011/02/10 06:00 [medline]
AID - JST.JSTAGE/analsci/26.1111 [pii]
AID - 10.2116/analsci.26.1111 [doi]
PST - ppublish
SO  - Anal Sci. 2010;26(10):1111-4. doi: 10.2116/analsci.26.1111.