PMID- 20953837
OWN - NLM
STAT- MEDLINE
DCOM- 20110427
LR  - 20211020
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 43
IP  - 2
DP  - 2011 Feb
TI  - Prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) 
      antigen-positive uterine tissues in gilts culled due to reproductive disturbance 
      in Thailand.
PG  - 451-7
LID - 10.1007/s11250-010-9713-0 [doi]
AB  - The objective of the present study was to determine the prevalence of porcine 
      reproductive and respiratory syndrome virus (PRRSV) antigen-positive uterine 
      tissue in gilts culled due to reproductive disturbance in relation to age at 
      culling, reasons for culling, herds, and PRRSV vaccination. Uterine tissues of 
      100 gilts from six swine herds in Thailand were collected. The 
      immunohistochemistry was performed to detect the PRRSV antigen using a 
      polymer-based non-avidin-biotin technique. PRRSV was detected in the cytoplasm of 
      the macrophages in the subepithelial connective tissue layers of the endometrium 
      in 33.0% of the culled gilts. The detection of PRRSV antigen varied among the 
      herds from 14.3% to 80.0% (P = 0.018). The detection of PRRSV in the uterine 
      tissues at different ages was not statistically different (29.6%, 39.4%, and 
      40.9% in gilts culled at 6-8, 9-10, and 11-16 months of age, respectively, P = 
      0.698), similar to the reasons for culling (P = 0.929). PRRSV antigen was found 
      in 24.5% of the gilts vaccinated against the EU-strain-modified-live PRRSV 
      vaccine and in 23.1% of the gilts vaccinated against the US-strain-modified-live 
      PRRSV (P = 0.941). The level of antibody titers against PRRSV had no impact on 
      PRRSV antigen detection in the uterine tissues. Similarly, the detection of PRRSV 
      antigen did not differ between the virgin gilts (35.4%) and the gilts mated 
      before culling (30.8%) (P = 0.622). It can be concluded that PRRSV remains in the 
      uterine tissue of the infected gilts for several months even though vaccinations 
      and acclimatization have been carried out.
FAU - Olanratmanee, Em-on
AU  - Olanratmanee EO
AD  - Department of Obstetrics, Gynecology, and Reproduction, Faculty of Veterinary 
      Science, Chulalongkorn University, Bangkok, Thailand 10330.
FAU - Wangnaitham, Supradit
AU  - Wangnaitham S
FAU - Thanawongnuwech, Roongroje
AU  - Thanawongnuwech R
FAU - Kunavongkrit, Annop
AU  - Kunavongkrit A
FAU - Tummaruk, Padet
AU  - Tummaruk P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101016
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (Antigens, Viral)
SB  - IM
MH  - Abortion, Veterinary
MH  - Anestrus
MH  - Animals
MH  - Antigens, Viral
MH  - Female
MH  - Infertility, Female/veterinary
MH  - Porcine Reproductive and Respiratory Syndrome/*epidemiology
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Swine
MH  - Thailand/epidemiology
MH  - Uterus/*virology
MH  - Vaginal Discharge/veterinary
EDAT- 2010/10/19 06:00
MHDA- 2011/04/28 06:00
CRDT- 2010/10/19 06:00
PHST- 2010/09/28 00:00 [accepted]
PHST- 2010/10/19 06:00 [entrez]
PHST- 2010/10/19 06:00 [pubmed]
PHST- 2011/04/28 06:00 [medline]
AID - 10.1007/s11250-010-9713-0 [doi]
PST - ppublish
SO  - Trop Anim Health Prod. 2011 Feb;43(2):451-7. doi: 10.1007/s11250-010-9713-0. Epub 
      2010 Oct 16.