PMID- 20953868
OWN - NLM
STAT- MEDLINE
DCOM- 20110427
LR  - 20211020
IS  - 1432-1017 (Electronic)
IS  - 0175-7571 (Linking)
VI  - 40
IP  - 2
DP  - 2011 Feb
TI  - Quantification of the effect of glycocalyx condition on membrane receptor 
      interactions using an acoustic wave sensor.
PG  - 209-15
LID - 10.1007/s00249-010-0632-9 [doi]
AB  - The effect of the cell glycocalyx on the binding of a membrane receptor, class I 
      major histocompatibility complex (MHC) human leukocyte antigen (HLA)-A2, to an 
      immobilized anti-HLA antibody was investigated using an acoustic sensor based on 
      a Love wave geometry. The enzyme neuraminidase was used to remove sialic acid 
      residues from the cell glycocalyx. Real-time measurements of the amplitude of the 
      acoustic wave showed that treatment with neuraminidase facilitates 
      HLA/anti-HLA-mediated cell attachment via a 3.6-fold increase of the 
      two-dimensional (2D) binding constant of the interaction. This could be 
      attributed to better approach of binding partners due to favorable condition of 
      the desialylated glycocalyx. The results underline the importance of 
      microtopological factors in membrane receptor binding and reveal the potential of 
      the Love wave sensor and 2D binding parameters for studying cell-substrate 
      binding events.
FAU - Saitakis, Michael
AU  - Saitakis M
AD  - Institute of Molecular Biology and Biotechnology, FO.R.T.H, 71110 Heraklion, 
      Greece.
FAU - Gizeli, Electra
AU  - Gizeli E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101017
PL  - Germany
TA  - Eur Biophys J
JT  - European biophysics journal : EBJ
JID - 8409413
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Acoustics/*instrumentation
MH  - Binding Sites
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Cell Membrane/*metabolism
MH  - Glycocalyx/*metabolism
MH  - HLA-A2 Antigen/chemistry/*metabolism
MH  - Humans
MH  - Kinetics
MH  - Receptors, Cell Surface/chemistry/*metabolism
EDAT- 2010/10/19 06:00
MHDA- 2011/04/28 06:00
CRDT- 2010/10/19 06:00
PHST- 2010/04/23 00:00 [received]
PHST- 2010/09/28 00:00 [accepted]
PHST- 2010/07/16 00:00 [revised]
PHST- 2010/10/19 06:00 [entrez]
PHST- 2010/10/19 06:00 [pubmed]
PHST- 2011/04/28 06:00 [medline]
AID - 10.1007/s00249-010-0632-9 [doi]
PST - ppublish
SO  - Eur Biophys J. 2011 Feb;40(2):209-15. doi: 10.1007/s00249-010-0632-9. Epub 2010 
      Oct 17.