PMID- 20954858
OWN - NLM
STAT- MEDLINE
DCOM- 20101112
LR  - 20161125
IS  - 1938-5404 (Electronic)
IS  - 0033-7587 (Linking)
VI  - 174
IP  - 5
DP  - 2010 Nov
TI  - A comparison of chromosome repair kinetics in G(0) and G(1) reveals that enhanced 
      repair fidelity under noncycling conditions accounts for increased potentially 
      lethal damage repair.
PG  - 566-73
LID - 10.1667/RR2159.1 [doi]
AB  - Potentially lethal damage (PLD) and its repair were studied in confluent human 
      fibroblasts by analyzing the kinetics of chromosome break rejoining and 
      misrejoining in irradiated cells that were either held in noncycling G(0) phase 
      or allowed to enter G(1) phase of the cell cycle immediately after 6 Gy 
      irradiation. Virally mediated premature chromosome condensation (PCC) methods 
      were combined with fluorescence in situ hybridization (FISH) to study chromosomal 
      aberrations in interphase. Flow cytometry revealed that the vast majority of 
      cells had not yet entered S phase 15 h after release from G(0). By this time some 
      95% of initially produced prematurely condensed chromosome breaks had rejoined, 
      indicating that most repair processes occurred during G(1). The rejoining 
      kinetics of prematurely condensed chromosome breaks was similar for each culture 
      condition. However, under noncycling conditions misrepair peaked at 0.55 
      exchanges per cell, while under cycling conditions (G(1)) it peaked at 1.1 
      exchanges per cell. At 12 h postirradiation, complex-type exchanges were 
      sevenfold more abundant for cycling cells (G(1)) than for noncycling cells 
      (G(0)). Since most repair in G(0)/G(1) occurs via the non-homologous end-joining 
      (NHEJ) process, increased PLD repair may result from improved cell cycle-specific 
      rejoining fidelity of the NHEJ pathway.
FAU - Liu, Cuihua
AU  - Liu C
AD  - a Department of Radiology, Graduate School of Medicine, Chiba University, Chiba, 
      Japan.
FAU - Kawata, Tetsuya
AU  - Kawata T
FAU - Shigematsu, Naoyuki
AU  - Shigematsu N
FAU - Cucinotta, Francis
AU  - Cucinotta F
FAU - George, Kerry
AU  - George K
FAU - Saito, Masayoshi
AU  - Saito M
FAU - Uno, Takashi
AU  - Uno T
FAU - Isobe, Kouichi
AU  - Isobe K
FAU - Ito, Hisao
AU  - Ito H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100917
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Cell Line
MH  - Cell Survival/genetics/radiation effects
MH  - Chromosome Breakage/radiation effects
MH  - Chromosomes/*genetics/radiation effects
MH  - DNA Damage
MH  - DNA Repair/*genetics/radiation effects
MH  - G1 Phase/*genetics/radiation effects
MH  - Humans
MH  - Infant, Newborn
MH  - Kinetics
MH  - Male
MH  - Resting Phase, Cell Cycle/*genetics/radiation effects
MH  - Time Factors
EDAT- 2010/10/20 06:00
MHDA- 2010/11/13 06:00
CRDT- 2010/10/20 06:00
PHST- 2010/10/20 06:00 [entrez]
PHST- 2010/10/20 06:00 [pubmed]
PHST- 2010/11/13 06:00 [medline]
AID - 10.1667/RR2159.1 [pii]
AID - 10.1667/RR2159.1 [doi]
PST - ppublish
SO  - Radiat Res. 2010 Nov;174(5):566-73. doi: 10.1667/RR2159.1. Epub 2010 Sep 17.