PMID- 20955719
OWN - NLM
STAT- MEDLINE
DCOM- 20120118
LR  - 20211020
IS  - 1873-7064 (Electronic)
IS  - 0028-3908 (Print)
IS  - 0028-3908 (Linking)
VI  - 60
IP  - 7-8
DP  - 2011 Jun
TI  - The 4-aminopyridine in vitro epilepsy model analyzed with a perforated 
      multi-electrode array.
PG  - 1142-53
LID - 10.1016/j.neuropharm.2010.10.007 [doi]
AB  - Epileptiform discharges recorded in the 4-aminopyridine (4-AP) in vitro epilepsy 
      model are mediated by glutamatergic and GABAergic signaling. Using a 60-channel 
      perforated multi-electrode array (pMEA) on corticohippocampal slices from 2 to 3 
      week old mice we recorded interictal- and ictal-like events. When glutamatergic 
      transmission was blocked, interictal-like events no longer initiated in the hilus 
      or CA3/CA1 pyramidal layers but originated from the dentate gyrus granule and 
      molecular layers. Furthermore, frequencies of interictal-like events were reduced 
      and durations were increased in these regions while cortical discharges were 
      completely blocked. Following GABA(A) receptor blockade interictal-like events no 
      longer propagated to the dentate gyrus while their frequency in CA3 increased; in 
      addition, ictal-like cortical events became shorter while increasing in 
      frequency. Lastly, drugs that affect tonic and synaptic GABAergic conductance 
      modulated the frequency, duration, initiation and propagation of interictal-like 
      events. These findings confirm and expand on previous studies indicating that 
      multiple synaptic mechanisms contribute to synchronize neuronal network activity 
      in forebrain structures. This article is part of a Special Issue entitled 'Trends 
      in neuropharmacology: in memory of Erminio Costa'.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Gonzalez-Sulser, Alfredo
AU  - Gonzalez-Sulser A
AD  - Interdisciplinary Program in Neuroscience, Georgetown University, Washington, DC, 
      USA.
FAU - Wang, Jing
AU  - Wang J
FAU - Motamedi, Gholam K
AU  - Motamedi GK
FAU - Avoli, Massimo
AU  - Avoli M
FAU - Vicini, Stefano
AU  - Vicini S
FAU - Dzakpasu, Rhonda
AU  - Dzakpasu R
LA  - eng
GR  - R01 NS047700/NS/NINDS NIH HHS/United States
GR  - R01 NS047700-13/NS/NINDS NIH HHS/United States
GR  - CAPMC/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101016
PL  - England
TA  - Neuropharmacology
JT  - Neuropharmacology
JID - 0236217
RN  - 0 (Anticonvulsants)
RN  - 0 (GABA-A Receptor Antagonists)
RN  - 0 (Isoxazoles)
RN  - 0 (Piperazines)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (Quinoxalines)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 118876-58-7 (2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 66016-70-4 (bicuculline methobromide)
RN  - 98Y1I8ZD4M (3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid)
RN  - BH3B64OKL9 (4-Aminopyridine)
RN  - K1M5RVL18S (gaboxadol)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
MH  - 4-Aminopyridine/*toxicity
MH  - Animals
MH  - Anticonvulsants/pharmacology
MH  - Bicuculline/analogs & derivatives/pharmacology
MH  - CA3 Region, Hippocampal/drug effects
MH  - *Disease Models, Animal
MH  - Electrodes
MH  - Epilepsy/*chemically induced/drug therapy
MH  - GABA-A Receptor Antagonists/pharmacology
MH  - Hippocampus/drug effects
MH  - In Vitro Techniques
MH  - Isoxazoles/pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microarray Analysis/*methods
MH  - Motion Pictures
MH  - Piperazines/pharmacology
MH  - Potassium Channel Blockers/*toxicity
MH  - Quinoxalines/pharmacology
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
MH  - Software
MH  - Somatosensory Cortex/drug effects
MH  - gamma-Aminobutyric Acid/metabolism
PMC - PMC3032005
MID - NIHMS247528
EDAT- 2010/10/20 06:00
MHDA- 2012/01/19 06:00
PMCR- 2012/06/01
CRDT- 2010/10/20 06:00
PHST- 2010/07/27 00:00 [received]
PHST- 2010/10/06 00:00 [revised]
PHST- 2010/10/12 00:00 [accepted]
PHST- 2010/10/20 06:00 [entrez]
PHST- 2010/10/20 06:00 [pubmed]
PHST- 2012/01/19 06:00 [medline]
PHST- 2012/06/01 00:00 [pmc-release]
AID - S0028-3908(10)00277-7 [pii]
AID - 10.1016/j.neuropharm.2010.10.007 [doi]
PST - ppublish
SO  - Neuropharmacology. 2011 Jun;60(7-8):1142-53. doi: 
      10.1016/j.neuropharm.2010.10.007. Epub 2010 Oct 16.