PMID- 20957522
OWN - NLM
STAT- MEDLINE
DCOM- 20110721
LR  - 20221207
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 43
IP  - 3
DP  - 2011 Mar
TI  - A proteomic investigation of B lymphocytes in an autistic family: a pilot study 
      of exposure to natural rubber latex (NRL) may lead to autism.
PG  - 443-52
LID - 10.1007/s12031-010-9463-5 [doi]
AB  - Autism is a multi-factorial neurodevelopmental disorder. We have investigated the 
      molecular mechanism involved in a Chinese family with autism by a proteomic 
      approach. Antibody chips containing 500 spots of human protein antibodies were 
      used to screen for differentially expressed proteins in the peripheral B 
      lymphocytes between autistic and non-autistic siblings in this family. Four 
      proteins relevant to immuno-pathway, including IKKalpha that was up-regulated and 
      Tyk2, EIF4G1 and PRKCI that were down-regulated, were identified differentially 
      expressed in autistic versus non-autistic siblings. Western blot analysis and 
      reverse transcription quantitative polymerase chain reaction validated the 
      differential expression of these four proteins. Based on the function of these 
      differentially expressed proteins, relevant studies on immunoglobulin E (IgE) 
      level, nuclear factor kappa B signaling activation and cell cycle were conducted 
      in both autistic and non-autistic children of this family. Considering the fact 
      that the family members were in close contact with natural rubber latex (NRL) and 
      that IgE-mediated cross-reactions could be triggered by Hevea brasiliensis 
      (Hev-b) proteins in NRL, we hypothesize that immune reactions triggered by close 
      contact with NRL might influence the functions of B lymphocytes by altering 
      expression of certain proteins identified in our experiments thus contributing to 
      the occurrence of autism.
FAU - Shen, Chen
AU  - Shen C
AD  - Peking University Center of Medical Genetics, Beijing, 100191, China.
FAU - Zhao, Xin-liang
AU  - Zhao XL
FAU - Ju, Weina
AU  - Ju W
FAU - Zou, Xiao-bing
AU  - Zou XB
FAU - Huo, Li-rong
AU  - Huo LR
FAU - Yan, Wu
AU  - Yan W
FAU - Zou, Jun-hua
AU  - Zou JH
FAU - Yan, Guo-di
AU  - Yan GD
FAU - Jenkins, Edmund C
AU  - Jenkins EC
FAU - Brown, W Ted
AU  - Brown WT
FAU - Zhong, Nanbert
AU  - Zhong N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101019
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Antibodies)
RN  - 0 (Latex)
RN  - 0 (Proteome)
RN  - 9006-04-6 (Rubber)
SB  - IM
MH  - Animals
MH  - Antibodies/analysis/immunology
MH  - Asian People
MH  - Autistic Disorder/*etiology/*immunology
MH  - B-Lymphocytes/cytology/*immunology/physiology
MH  - Cell Cycle/physiology
MH  - Cell Line
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Hevea/chemistry/immunology
MH  - Humans
MH  - Latex/*immunology
MH  - Latex Hypersensitivity/*immunology
MH  - Male
MH  - Pedigree
MH  - Pilot Projects
MH  - Protein Array Analysis
MH  - Proteome/*analysis
MH  - Rubber/*chemistry
EDAT- 2010/10/20 06:00
MHDA- 2011/07/22 06:00
CRDT- 2010/10/20 06:00
PHST- 2010/08/24 00:00 [received]
PHST- 2010/10/05 00:00 [accepted]
PHST- 2010/10/20 06:00 [entrez]
PHST- 2010/10/20 06:00 [pubmed]
PHST- 2011/07/22 06:00 [medline]
AID - 10.1007/s12031-010-9463-5 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2011 Mar;43(3):443-52. doi: 10.1007/s12031-010-9463-5. Epub 2010 
      Oct 19.