PMID- 20960180
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20211020
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 25
IP  - 5
DP  - 2010 Oct
TI  - In vitro anti-influenza virus activities of sulfated polysaccharide fractions 
      from Gracilaria lemaneiformis.
PG  - 341-51
LID - 10.1007/s12250-010-3137-x [doi]
AB  - In this paper, in vitro anti-influenza virus activities of sulfated 
      polysaccharide fractions from Gracilaria lemaneiformis were investigated. 
      Cytotoxicities and antiviral activities of Gracilaria lemaneiformis 
      polysaccharides (PGL), Gracilaria lemaneiformis polysaccharide fraction-1 (GL-1), 
      Gracilaria lemaneiformis polysaccharide fraction-2 (GL-2) and Gracilaria 
      lemaneiformis polysaccharide fraction-3 (GL-3) were studied by the Methyl 
      thiazolyl tetrazolium (MTT) method, and the inhibitory effect against Human 
      influenza virus H1-364 induced cytopathic effect (CPE) on MDCK cells were 
      observed by the CPE method. In addition, the antiviral mechanism of PGL was 
      explored by Plaque forming unit (PFU), MTT and CPE methods. The results showed: 
      i) Cytotoxicities were not significantly revealed, and H1-364 induced CPE was 
      also reduced treated with sulfated polysaccharide fractions from Gracilaria 
      lemaneiformis; ii) Antiviral activities were associated with the mass percentage 
      content of sulfate groups in polysaccharide fractions, which was about 13%, in 
      polysaccharides (PGL and GL-2) both of which exhibited higher antiviral activity; 
      iii) A potential antiviral mechanism to explain these observations is that viral 
      adsorption and replication on host cells were inhibited by sulfated 
      polysaccharides from Gracilaria lemaneiformis. In conclusion, Anti-influenza 
      virus activities of sulfated polysaccharide fractions from Gracilaria 
      lemaneiformis were revealed, and the antiviral activities were associated with 
      content of sulfate groups in polysaccharide fractions.
FAU - Chen, Mei-Zhen
AU  - Chen MZ
AD  - Department of Biology of Shantou University, Shantou, 515063, China. 
      chenmz@stu.edu.cn
FAU - Xie, Hao-Gui
AU  - Xie HG
FAU - Yang, La-Wei
AU  - Yang LW
FAU - Liao, Zao-Hui
AU  - Liao ZH
FAU - Yu, Jie
AU  - Yu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101008
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
RN  - 0 (Antiviral Agents)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
RN  - 0 (Sulfates)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/isolation & purification/*pharmacology/toxicity
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cytopathogenic Effect, Viral/drug effects
MH  - Dogs
MH  - Gracilaria/*chemistry
MH  - Humans
MH  - Influenza, Human/virology
MH  - Orthomyxoviridae/*drug effects/isolation & purification
MH  - Plant Extracts/isolation & purification/*pharmacology/toxicity
MH  - Polysaccharides/isolation & purification/*pharmacology/toxicity
MH  - Sulfates/isolation & purification/pharmacology/toxicity
MH  - Tetrazolium Salts/metabolism
MH  - Thiazoles/metabolism
MH  - Viral Plaque Assay
PMC - PMC8227872
EDAT- 2010/10/21 06:00
MHDA- 2011/01/25 06:00
PMCR- 2011/10/08
CRDT- 2010/10/21 06:00
PHST- 2010/03/22 00:00 [received]
PHST- 2010/07/07 00:00 [accepted]
PHST- 2010/10/21 06:00 [entrez]
PHST- 2010/10/21 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
PHST- 2011/10/08 00:00 [pmc-release]
AID - 3137 [pii]
AID - 10.1007/s12250-010-3137-x [doi]
PST - ppublish
SO  - Virol Sin. 2010 Oct;25(5):341-51. doi: 10.1007/s12250-010-3137-x. Epub 2010 Oct 
      8.