PMID- 20960296
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20211020
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 25
IP  - 3
DP  - 2010 Jun
TI  - Complete genomic sequence of transmissible gastroenteritis virus TS and 3' end 
      sequence characterization following cell culture.
PG  - 213-24
LID - 10.1007/s12250-010-3108-2 [doi]
AB  - The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain 
      TS, previously isolated from Gansu province, was cloned and compared with 
      published sequence data from other TGEV strains. Phylogenetic tree analysis based 
      on the amino acid and nucleotide sequences of the S gene showed that the TGEV 
      strains were divided into 3 clusters. TGEV TS showed a close evolutionary 
      relationship to the American Miller cluster but had a 5' non-translated region 
      (NTR) sequence closely related to the American Purdue cluster. Continued culture 
      in different cell types indicated that TGEV TS virulence could be attenuated 
      after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney 
      cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS.
FAU - Li, Jian-qiang
AU  - Li JQ
AD  - Key laboratory of Animal Virology of Ministry of Agriculture, State Key 
      Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research 
      Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
FAU - Cheng, Jie
AU  - Cheng J
FAU - Lan, Xi
AU  - Lan X
FAU - Li, Xue-rui
AU  - Li XR
FAU - Li, Wei
AU  - Li W
FAU - Yin, Xiang-ping
AU  - Yin XP
FAU - Li, Bao-yu
AU  - Li BY
FAU - Yang, Bin
AU  - Yang B
FAU - Li, Zhi-yong
AU  - Li ZY
FAU - Zhang, Yun
AU  - Zhang Y
FAU - Liu, Ji-xing
AU  - Liu JX
LA  - eng
SI  - GENBANK/DQ201447
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100606
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
RN  - 0 (3' Untranslated Regions)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - *3' Untranslated Regions
MH  - Animals
MH  - Cell Line
MH  - Cluster Analysis
MH  - *Genome, Viral
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Viral/*genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Sequence Homology, Nucleic Acid
MH  - Serial Passage
MH  - Swine
MH  - Transmissible gastroenteritis virus/*genetics/pathogenicity
MH  - Virulence
PMC - PMC7090398
EDAT- 2010/10/21 06:00
MHDA- 2011/01/25 06:00
PMCR- 2011/06/06
CRDT- 2010/10/21 06:00
PHST- 2009/10/29 00:00 [received]
PHST- 2010/04/14 00:00 [accepted]
PHST- 2010/10/21 06:00 [entrez]
PHST- 2010/10/21 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
PHST- 2011/06/06 00:00 [pmc-release]
AID - 3108 [pii]
AID - 10.1007/s12250-010-3108-2 [doi]
PST - ppublish
SO  - Virol Sin. 2010 Jun;25(3):213-24. doi: 10.1007/s12250-010-3108-2. Epub 2010 Jun 
      6.