PMID- 20961941
OWN - NLM
STAT- MEDLINE
DCOM- 20110426
LR  - 20191210
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 26
IP  - 1
DP  - 2011 Jan
TI  - In vitro maturation of human germinal vesicle-stage oocytes: role of epidermal 
      growth factor-like growth factors in the culture medium.
PG  - 76-81
LID - 10.1093/humrep/deq290 [doi]
AB  - BACKGROUND: In vitro maturation (IVM) of oocytes is a promising technique to 
      reduce the costs and avert the side effects of gonadotrophin stimulation for IVF. 
      The pregnancy rates from oocytes matured in vitro are still lower than those of 
      in vivo stimulation cycles, indicating that optimization of IVM remains a 
      challenge. Recently, it was demonstrated that LH exerts its action on ovulation, 
      at least in part, through stimulation of the production of the epidermal growth 
      factor family members amphiregulin (Areg) and epiregulin (Ereg) in pre-ovulatory 
      follicles, and they, in turn, serve as paracrine mediators of LH. We aimed to 
      investigate the effect of supplementation of the medium with Areg and Ereg on the 
      maturation rate of immature oocytes. METHODS: A total of 105 sibling human 
      germinal vesicle (GV) oocytes obtained after gonadotrophin stimulation were 
      cultured in a complex defined medium either with or without supplemented 
      recombinant human Areg (75 ng/ml) and Ereg (75 ng/ml) for 24 h. RESULTS: 
      Significantly more oocytes reached the metaphase II stage at 24 h in media 
      supplemented with Areg and Ereg (75.5 versus 36.5%, P < 0.001). In vitro matured 
      oocytes retrieved from the two subgroups had no statistically significant 
      difference in fertilization and cleavage rates or morphology scores. Overall, a 
      significantly higher number of Day 2 (52.8 versus 26.9% P < 0.01) and Day 3 (45.2 
      versus 23%, P < 0.05) embryos originated from GV oocytes cultured in the Areg- 
      and Ereg-enriched medium. CONCLUSIONS: Supplementation of the maturation medium 
      with Areg and Ereg improves the maturation of human GV oocytes in vitro.
FAU - Ben-Ami, I
AU  - Ben-Ami I
AD  - Department of Obstetrics and Gynecology, Assaf Harofeh Medical Center, Tel Aviv 
      University, Zerifin, Israel. idorit@netvision.net.il
FAU - Komsky, A
AU  - Komsky A
FAU - Bern, O
AU  - Bern O
FAU - Kasterstein, E
AU  - Kasterstein E
FAU - Komarovsky, D
AU  - Komarovsky D
FAU - Ron-El, R
AU  - Ron-El R
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20101020
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (EREG protein, human)
RN  - 0 (Epiregulin)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Adult
MH  - Amphiregulin
MH  - Cell Culture Techniques
MH  - EGF Family of Proteins
MH  - Epidermal Growth Factor/*pharmacology
MH  - Epiregulin
MH  - Female
MH  - Glycoproteins/*pharmacology
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*pharmacology
MH  - Oocytes/*drug effects/growth & development
MH  - Prospective Studies
MH  - Reproductive Techniques, Assisted
MH  - Sperm Injections, Intracytoplasmic
EDAT- 2010/10/22 06:00
MHDA- 2011/04/27 06:00
CRDT- 2010/10/22 06:00
PHST- 2010/10/22 06:00 [entrez]
PHST- 2010/10/22 06:00 [pubmed]
PHST- 2011/04/27 06:00 [medline]
AID - deq290 [pii]
AID - 10.1093/humrep/deq290 [doi]
PST - ppublish
SO  - Hum Reprod. 2011 Jan;26(1):76-81. doi: 10.1093/humrep/deq290. Epub 2010 Oct 20.