PMID- 20965240
OWN - NLM
STAT- MEDLINE
DCOM- 20110526
LR  - 20131121
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 133
IP  - 2
DP  - 2011 Jan 27
TI  - Antidiabetic activity of alcoholic stem extract of Nervilia plicata in 
      streptozotocin-nicotinamide induced type 2 diabetic rats.
PG  - 480-3
LID - 10.1016/j.jep.2010.10.025 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Nervilia plicata (Orchidaceae) has long been used 
      in the antidiabetic medicinal preparations of traditional healers of Wayanad 
      (Kerala), but recuperative potential of the plant was remained undefined. We 
      demonstrated the regenerative potential of the plant extract on kidney affected 
      by type 2 diabetes besides lowering blood glucose. AIM OF THE STUDY: The aim of 
      the current study was to investigate the recuperative and regenerative potential 
      of alcoholic stem extract of Nervilia plicata on streptozotocin-nicotinamide 
      induced type 2 diabetic models. MATERIALS AND METHODS: Non insulin dependent 
      diabetes mellitus (NIDDM) was induced in overnight fasted rats by intramuscular 
      injection (IMI) of 60 mg/kg STZ and 120 mg/kg of nicotinamide after 5 min 
      interval. Blood glucose was assessed by a glucometer, serum urea and creatinine 
      levels were determined by diacetylmonooxime method and Jaffe reaction 
      respectively. Kidney sections were taken and stained with Masson's tri-dye and 
      Periodic Acid Schiff (PAS) and examined for structural changes. Also lipid 
      peroxidation product (LPP) levels were determined as thio barbituric acid 
      reactive substance levels (TBARS) method. RESULTS: On administration of 5 mg/kg 
      of plant extract, blood glucose levels of the NIDDM rats showed 62.00 and 76.29% 
      decrease in the blood glucose levels on day 0 and day 30 respectively. Damages 
      caused to the kidney tissue were negligible or not seen. Serum urea and 
      creatinine levels showed 61.49 and 70.96% decrease on day 30. LPP levels of 
      kidney and pancreas showed 70.58 and 77.41% decrease respectively. CONCLUSION: 
      These results demonstrate significant antidiabetic and regenerative potential of 
      the Nervilia plicata, justifying the use of plant in the indigenous system of 
      medicine. Isolation and characterisation of the compound(s) playing pivotal role 
      in the cure would open new vistas in the therapy of type 2 diabetes.
CI  - Copyright A(c) 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Kumar, E K Dilip
AU  - Kumar EK
AD  - Phytopharmacology Laboratory, Department of Studies in Botany, University of 
      Mysore, Manasagangotri, Mysore 570006, Karnataka, India.
FAU - Janardhana, G R
AU  - Janardhana GR
LA  - eng
PT  - Journal Article
DEP - 20101019
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Plant Extracts)
RN  - 3K9958V90M (Ethanol)
RN  - 8W8T17847W (Urea)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - Creatinine/blood
MH  - Diabetes Mellitus, Experimental/blood/*drug therapy/pathology
MH  - Ethanol
MH  - Ethnopharmacology
MH  - Female
MH  - Hypoglycemic Agents/isolation & purification/*pharmacology
MH  - India
MH  - Kidney/drug effects/metabolism/pathology
MH  - Lipid Peroxidation/drug effects
MH  - Male
MH  - Medicine, Traditional
MH  - *Orchidaceae/chemistry
MH  - Phytotherapy
MH  - Plant Extracts/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Urea/blood
EDAT- 2010/10/23 06:00
MHDA- 2011/05/27 06:00
CRDT- 2010/10/23 06:00
PHST- 2009/02/02 00:00 [received]
PHST- 2010/08/13 00:00 [revised]
PHST- 2010/10/11 00:00 [accepted]
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2011/05/27 06:00 [medline]
AID - S0378-8741(10)00717-8 [pii]
AID - 10.1016/j.jep.2010.10.025 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2011 Jan 27;133(2):480-3. doi: 10.1016/j.jep.2010.10.025. Epub 
      2010 Oct 19.