PMID- 20965747
OWN - NLM
STAT- MEDLINE
DCOM- 20110606
LR  - 20211020
IS  - 1095-8320 (Electronic)
IS  - 1045-1056 (Print)
IS  - 1045-1056 (Linking)
VI  - 39
IP  - 1
DP  - 2011 Jan
TI  - Effect of storage conditions on the stability of recombinant human MCP-1/CCL2.
PG  - 29-32
LID - 10.1016/j.biologicals.2010.09.003 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) is commercially available in a form of 
      recombinant protein. This makes it more convenient to study the functions of 
      MCP-1 and its involvement in many cell functions. However, when using MCP-1 in 
      experimental studies, if the analysis is not performed immediately, the stability 
      of recombinant MCP-1 may become an issue. In this study, the stability of 
      recombinant MCP-1 at different concentrations and storage conditions was 
      investigated. Results show that no significant loss of MCP-1 is observed when 
      MCP-1 solutions were stored at non-freezing condition (4  degrees C) for seven days. 
      However, for storage at freezing conditions (-20  degrees C or -81  degrees C), it appears that 
      the first freeze-thaw cycle may contribute to some loss of MCP-1 in the 
      solutions, and such loss may be concentration and time dependent. The effect of 
      multiple freeze-thaw cycles for storage at freezing conditions was also examined. 
      Data reveal that the second freeze-thaw cycle causes approximately 50% loss of 
      MCP-1 in the solutions. This finding confirms that multiple freeze-thaw cycles 
      should be avoided. The findings of this study provide an outline of how storage 
      can affect the stability of recombinant proteins and should be taken into account 
      during the evaluation of the concentration of recombinant proteins.
CI  - Copyright (c) 2010 The International Association for Biologicals. Published by 
      Elsevier Ltd. All rights reserved.
FAU - Leemasawatdigul, Krisada
AU  - Leemasawatdigul K
AD  - School of Chemical Engineering, Oklahoma State University, 423 Engineering North, 
      Stillwater, OK 74078, USA. leemasa@okstate.edu
FAU - Gappa-Fahlenkamp, Heather
AU  - Gappa-Fahlenkamp H
LA  - eng
GR  - R15 EB009527/EB/NIBIB NIH HHS/United States
GR  - R15 EB009527-01/EB/NIBIB NIH HHS/United States
GR  - 1R15EB009527-01/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20101020
PL  - England
TA  - Biologicals
JT  - Biologicals : journal of the International Association of Biological 
      Standardization
JID - 9004494
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Chemokine CCL2/*chemistry/genetics/metabolism
MH  - Drug Stability
MH  - Drug Storage/methods/standards
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Freezing
MH  - Humans
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Reproducibility of Results
MH  - Technology, Pharmaceutical/*methods/standards
PMC - PMC3025303
MID - NIHMS240630
EDAT- 2010/10/23 06:00
MHDA- 2011/06/07 06:00
PMCR- 2012/01/01
CRDT- 2010/10/23 06:00
PHST- 2010/07/09 00:00 [received]
PHST- 2010/09/16 00:00 [revised]
PHST- 2010/09/17 00:00 [accepted]
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2011/06/07 06:00 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - S1045-1056(10)00165-X [pii]
AID - 10.1016/j.biologicals.2010.09.003 [doi]
PST - ppublish
SO  - Biologicals. 2011 Jan;39(1):29-32. doi: 10.1016/j.biologicals.2010.09.003. Epub 
      2010 Oct 20.