PMID- 20965833
OWN - NLM
STAT- MEDLINE
DCOM- 20130211
LR  - 20131121
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 30
IP  - 10
DP  - 2010 Oct
TI  - [High glucose induces INS-1 cell apoptosis by activating nuclear factor-kappaB].
PG  - 2307-9
AB  - OBJECTIVE: To study of the role of nuclear transcription factor-kappaB (NF-kappaB) in 
      high glucose-induced apoptosis in INS-1 cells. METHODS: Rat insulinoma (INS-1) 
      cells cultured in RPMI 1640 medium were treated with 11.1 mmol/L glucose, 33.3 
      mmol/L glucose, or 33.3 mmol/L glucose plus 5 micromol/L NF-kappaB inhibitors for 48 h. 
      The expression of NF-kappaB subunit P65 protein in the cell nuclei was detected by 
      Western blotting, IKK belta mRNA level by quantitative RT-PCR, and cell apoptosis 
      by Annexin V-PI double staining. RESULTS: Compared with the control levels, IKK 
      belta mRNA levels of the cells significantly increased in response to 33.3 mmol/L 
      glucose exposure (P<0.01), which also resulted in significantly increased P65 
      protein expression in the cell nuclei (P<0.01) and cell apoptosis rate (P<0.05). 
      Compared with those in the high glucose group, the expression of IKK belta mRNA 
      and P65 protein and cell apoptosis rate decreased significantly after treatment 
      with 33.3 mmol/L glucose plus 5 micromol/L NF-kappaB inhibitors (P<0.05). CONCLUSION: 
      High glucose induces NF-kappaB activation in INS-1 cells, and inhibition of NF-kappaB 
      activation may protect INS-1 cells from high glucose-induced cell apoptosis.
FAU - Zhang, Qiao-ling
AU  - Zhang QL
AD  - Department of Endocrinology, Nanfang Hospital, Southern Medical University, 
      Guangzhou510515, China. qiaoling000@yahoo.com.cn
FAU - Xue, Yao-ming
AU  - Xue YM
FAU - Zhu, Bo
AU  - Zhu B
FAU - Li, Jia
AU  - Li J
FAU - Sha, Jian-ping
AU  - Sha JP
FAU - Li, Sheng-jian
AU  - Li SJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Rela protein, rat)
RN  - 0 (Transcription Factor RelA)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Line, Tumor
MH  - Gene Expression Regulation
MH  - Glucose/*adverse effects/metabolism
MH  - Insulinoma/*pathology
MH  - Pancreatic Neoplasms/*pathology
MH  - Rats
MH  - Transcription Factor RelA/*metabolism
EDAT- 2010/10/23 06:00
MHDA- 2013/02/12 06:00
CRDT- 2010/10/23 06:00
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2013/02/12 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2010 Oct;30(10):2307-9.