PMID- 20965948
OWN - NLM
STAT- MEDLINE
DCOM- 20110304
LR  - 20131121
IS  - 1753-9455 (Electronic)
IS  - 1753-9447 (Linking)
VI  - 4
IP  - 6
DP  - 2010 Dec
TI  - Exercise improves hemodynamic profiles and increases red blood cell 
      concentrations of purine nucleotides in a rodent model.
PG  - 341-7
LID - 10.1177/1753944710387809 [doi]
AB  - OBJECTIVE: To study the effect of exercise on hemodynamic profiles and red blood 
      cell (RBC) concentrations of adenosine-5'-triphosphate (ATP). METHODS: Male 
      Sprague-Dawley (SD) rats (n = 9) were exercised on a treadmill for 15 min at a 
      speed of 10 m/min with a 5% gradient after an hour settling down in a restrainer. 
      Blood samples were collected via an indwelling carotid artery catheter using a 
      'Stopping Solution' from each rat before, during and after exercise. Hemodynamic 
      recordings were collected continuously throughout the experiment. Concentrations 
      of ATP and other purine nucleotides in the RBCs were determined by a validated 
      high-performance liquid chromatography (HPLC) assay. A control group (n = 12) was 
      treated the same way except without the exercise. Data between groups were 
      analyzed by analysis of variance (ANOVA) and Student's t-test, and differences 
      were considered significant when p < 0.05. RESULTS: Exercise increased systolic 
      blood pressure (SBP; 141+/-23 vs. 132+/-17 mmHg) and heart rate (HR; 420+/-33 vs. 
      397+/-41 bpm), but decreased diastolic blood pressure (DBP; 105+/-18 vs. 110+/-14 
      mmHg). This was followed by a postexercise condition when SBP, DBP, and HR were 
      decreased for the remainder of the experiment. RBC concentrations of ATP and 
      guanosine-5'-triphosphate (GTP) increased significantly during exercise and 
      continued to increase for 5 hours postexercise (1.5+/-0.75 vs. 0.96 vs. 0.36 mM for 
      ATP; and 0.14+/-0.061 vs. 0.058+/-0.030 mM) (p < 0.05). CONCLUSION: Exercise 
      increased RBC ATP concentrations in a rodent model, which was correlated with the 
      decrease in BP and HR postexercise.
FAU - Yeung, Pollen K F
AU  - Yeung PK
AD  - 5968 College Street, Burbidge Building Dalhousie University, Halifax, NS, Canada 
      B3H 3J5. pollen.yeung@dal.ca
FAU - Dauphinee, Julie
AU  - Dauphinee J
FAU - Gouzoules, Thera
AU  - Gouzoules T
FAU - Simonson, Krista
AU  - Simonson K
FAU - Schindler, Christoph
AU  - Schindler C
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101021
PL  - England
TA  - Ther Adv Cardiovasc Dis
JT  - Therapeutic advances in cardiovascular disease
JID - 101316343
RN  - 0 (Purine Nucleotides)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/*metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Blood Pressure/physiology
MH  - Chromatography, High Pressure Liquid
MH  - Erythrocytes/*metabolism
MH  - Guanosine Triphosphate/metabolism
MH  - Heart Rate/physiology
MH  - Male
MH  - Physical Conditioning, Animal/*physiology
MH  - Purine Nucleotides/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2010/10/23 06:00
MHDA- 2011/03/05 06:00
CRDT- 2010/10/23 06:00
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2011/03/05 06:00 [medline]
AID - 1753944710387809 [pii]
AID - 10.1177/1753944710387809 [doi]
PST - ppublish
SO  - Ther Adv Cardiovasc Dis. 2010 Dec;4(6):341-7. doi: 10.1177/1753944710387809. Epub 
      2010 Oct 21.