PMID- 20966538
OWN - NLM
STAT- MEDLINE
DCOM- 20110425
LR  - 20110105
IS  - 1741-2552 (Electronic)
IS  - 1741-2552 (Linking)
VI  - 7
IP  - 6
DP  - 2010 Dec
TI  - A composite SWNT-collagen matrix: characterization and preliminary assessment as 
      a conductive peripheral nerve regeneration matrix.
PG  - 066002
LID - 10.1088/1741-2560/7/6/066002 [doi]
AB  - Unique in their structure and function, single-walled carbon nanotubes (SWNTs) 
      have received significant attention due to their potential to create unique 
      conductive materials. For neural applications, these conductive materials hold 
      promise as they may enhance regenerative processes. However, like other 
      nano-scaled biomaterials it is important to have a comprehensive understanding 
      how these materials interact with cell systems and how the biological system 
      responds to their presence. These investigations aim to further our understanding 
      of SWNT-cell interactions by assessing the effect SWNT/collagen hydrogels have on 
      PC12 neuronal-like cells seeded within and (independently) on top of the 
      composite material. Two types of collagen hydrogels were prepared: (1) SWNTs 
      dispersed directly within the collagen (SWNT/COL) and (2) albumin-coated SWNTs 
      prepared using the surfactant 'sodium cholate' to improve dispersion 
      (AL-SWNT/COL) and collagen alone serving as a control (COL). SWNT dispersion was 
      significantly improved when using surfactant-assisted dispersion. The enhanced 
      dispersion resulted in a stiffer, more conductive material with an increased 
      collagen fiber diameter. Short-term cell interactions with PC12 cells and SWNT 
      composites have shown a stimulatory effect on cell proliferation relative to 
      plain collagen controls. In parallel to these results, p53 gene displayed normal 
      expression levels, which indicates the absence of nanoparticle-induced DNA 
      damage. In summary, these mechanically tunable SWNT-collagen scaffolds show the 
      potential for enhanced electrical activity and have shown positive in vitro 
      biocompatibility results offering further evidence that SWNT-based materials have 
      an important role in promoting neuronal regeneration.
FAU - Tosun, Z
AU  - Tosun Z
AD  - J Crayton Pruitt Family Department of Biomedical Engineering, University of 
      Florida, Gainesville, FL, USA.
FAU - McFetridge, P S
AU  - McFetridge PS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101022
PL  - England
TA  - J Neural Eng
JT  - Journal of neural engineering
JID - 101217933
RN  - 0 (Biocompatible Materials)
RN  - 0 (Hydrogels)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Nerve Tissue Proteins)
RN  - 63231-63-0 (RNA)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - Cell Count
MH  - Cell Survival
MH  - Collagen/*chemistry
MH  - Electric Conductivity
MH  - Electrophysiological Phenomena
MH  - Hydrogels
MH  - Mechanical Phenomena
MH  - Microscopy, Electron, Scanning
MH  - Nanotubes, Carbon/*chemistry
MH  - Nerve Regeneration/*physiology
MH  - Nerve Tissue Proteins/biosynthesis
MH  - Neural Conduction/physiology
MH  - PC12 Cells
MH  - Peripheral Nerves/*physiology
MH  - RNA/biosynthesis/isolation & purification
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spectrum Analysis, Raman
EDAT- 2010/10/23 06:00
MHDA- 2011/04/26 06:00
CRDT- 2010/10/23 06:00
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2011/04/26 06:00 [medline]
AID - S1741-2560(10)65374-8 [pii]
AID - 10.1088/1741-2560/7/6/066002 [doi]
PST - ppublish
SO  - J Neural Eng. 2010 Dec;7(6):066002. doi: 10.1088/1741-2560/7/6/066002. Epub 2010 
      Oct 22.