PMID- 20967895
OWN - NLM
STAT- MEDLINE
DCOM- 20110331
LR  - 20101224
IS  - 1097-0061 (Electronic)
IS  - 0749-503X (Linking)
VI  - 28
IP  - 1
DP  - 2011 Jan
TI  - Chlorophenol stress affects aromatic amino acid biosynthesis-a genome-wide study.
PG  - 81-91
LID - 10.1002/yea.1825 [doi]
AB  - Chlorophenols are a class of chemicals commonly used in preservatives, 
      disinfectants, algaecides, herbicides and pesticides. However, there is a growing 
      evidence that these compounds are a threat to human health. This is alarming as 
      many chlorophenols are common pollutants found in the global environment at 
      potentially biohazardous levels. Despite chlorophenols being abundant, widely 
      used and poisonous, we know relatively little about their mechanism of toxicity 
      in eukaryotes. Thus, we performed genome-wide growth screens using Saccharomyces 
      cerevisiae to understand the molecular basis of chlorophenol toxicity. Of  approximately 4850 
      single gene knockout strains tested, 393 mutants showed growth sensitivity to 
      treatment with 4-chlorophenol (4-CP), 2,4-dichlorophenol (2,4-DCP) or 
      pentachlorophenol (PCP). Only eight mutants showed growth hypersensitivity to all 
      the three treatments and harboured deletions in genes important for aromatic 
      amino acid biosynthesis (ARO1, ARO7) or mitochondrial protein synthesis and 
      respiration (ATP5, ISA1, RML2, GET2, SLS1, MRPL38).
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Yadav, Vikas
AU  - Yadav V
AD  - Institute of Genomics and Integrative Biology (IGIB), CSIR, Delhi, India.
FAU - Shitiz, Kirti
AU  - Shitiz K
FAU - Pandey, Rishi
AU  - Pandey R
FAU - Yadav, Jyoti
AU  - Yadav J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101022
PL  - England
TA  - Yeast
JT  - Yeast (Chichester, England)
JID - 8607637
RN  - 0 (Amino Acids, Aromatic)
RN  - 0 (Chlorophenols)
RN  - 0 (Disinfectants)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Herbicides)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Pesticides)
SB  - IM
MH  - Amino Acids, Aromatic/*biosynthesis
MH  - Chlorophenols/*toxicity
MH  - Disinfectants/metabolism
MH  - Environmental Pollutants/metabolism/toxicity
MH  - *Genome, Fungal
MH  - Herbicides/metabolism
MH  - Mitochondrial Proteins/*biosynthesis
MH  - Pesticides/metabolism
MH  - Phenotype
MH  - Saccharomyces cerevisiae/*drug effects/genetics
MH  - Sequence Deletion
EDAT- 2010/10/23 06:00
MHDA- 2011/04/01 06:00
CRDT- 2010/10/23 06:00
PHST- 2010/06/17 00:00 [received]
PHST- 2010/09/07 00:00 [accepted]
PHST- 2010/10/23 06:00 [entrez]
PHST- 2010/10/23 06:00 [pubmed]
PHST- 2011/04/01 06:00 [medline]
AID - 10.1002/yea.1825 [doi]
PST - ppublish
SO  - Yeast. 2011 Jan;28(1):81-91. doi: 10.1002/yea.1825. Epub 2010 Oct 22.