PMID- 20969864
OWN - NLM
STAT- MEDLINE
DCOM- 20110204
LR  - 20161125
IS  - 1090-2430 (Electronic)
IS  - 0014-4886 (Linking)
VI  - 227
IP  - 1
DP  - 2011 Jan
TI  - Survival and differentiation of neuroectodermal cells with stem cell properties 
      at different oxygen levels.
PG  - 136-48
LID - 10.1016/j.expneurol.2010.10.004 [doi]
AB  - Freeze-lesioned regions of the forebrain cortex provide adequate environment for 
      growth of non-differentiated neural progenitors, but do not support their neuron 
      formation. Reduced oxygen supply, among numerous factors, was suspected to impair 
      neuronal cell fate commitment. In the present study, proliferation and 
      differentiation of neural stem/progenitor cells were investigated at different 
      oxygen levels both in vitro and in vivo. Low (1% atmospheric) oxygen supply did 
      not affect the in vitro viability and proliferation of stem cells or the 
      transcription of "stemness" genes but impaired the viability of committed 
      neuronal progenitors and the expression of proneural and neuronal genes. 
      Consequently, the rate of in vitro neuron formation was markedly reduced under 
      hypoxic conditions. In vivo, neural stem/progenitor cells survived and 
      proliferated in freeze-lesioned adult mouse forebrains, but did not develop into 
      neurons. Hypoperfusion-caused hypoxia in lesioned cortices was partially 
      corrected by hyperbaric oxygen treatment (HBOT). HBOT, while reduced the rate of 
      cell proliferation at the lesion site, resulted in sporadic neuron formation from 
      implanted neural stem cells. The data indicate that in hypoxic brain areas, 
      neural stem cells survive and proliferate, but neural tissue-type differentiation 
      can not proceed. Oxygenation renders the damaged brain areas more permissive for 
      tissue-type differentiation and may help the integration of neural 
      stem/progenitor cells.
CI  - 2010 Elsevier Inc. All rights reserved.
FAU - Zadori, Anita
AU  - Zadori A
AD  - Laboratory of Cellular and Developmental Neurobiology, Institute of Experimental 
      Medicine, Hungarian Academy of Sciences, Budapest 1083 Szigony u. 43, Budapest, 
      Hungary.
FAU - Agoston, Viktor Antal
AU  - Agoston VA
FAU - Demeter, Kornel
AU  - Demeter K
FAU - Hadinger, Nora
AU  - Hadinger N
FAU - Varady, Linda
AU  - Varady L
FAU - Kohidi, Timea
AU  - Kohidi T
FAU - Gobl, Anna
AU  - Gobl A
FAU - Nagy, Zoltan
AU  - Nagy Z
FAU - Madarasz, Emilia
AU  - Madarasz E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101020
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Hif1a protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nanog Homeobox Protein)
RN  - 0 (Nanog protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (SOXB1 Transcription Factors)
RN  - 0 (Sox2 protein, mouse)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 5688UTC01R (Tretinoin)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Behavior, Animal
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cell Line, Tumor
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects/physiology
MH  - Cell Transplantation/physiology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/drug effects/physiology
MH  - Green Fluorescent Proteins/genetics
MH  - Homeodomain Proteins/metabolism
MH  - Hyperbaric Oxygenation/methods
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Hypoxia-Ischemia, Brain/pathology/surgery
MH  - Locomotion/physiology
MH  - Male
MH  - Mice
MH  - Models, Biological
MH  - Nanog Homeobox Protein
MH  - Nerve Tissue Proteins/metabolism
MH  - Neural Plate/cytology
MH  - Neuroepithelial Cells/*drug effects
MH  - Oxygen/metabolism/*pharmacology
MH  - SOXB1 Transcription Factors/metabolism
MH  - Stem Cells/drug effects/*physiology
MH  - Time Factors
MH  - Transfection/methods
MH  - Tretinoin/pharmacology
EDAT- 2010/10/26 06:00
MHDA- 2011/02/05 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/04/15 00:00 [received]
PHST- 2010/10/06 00:00 [revised]
PHST- 2010/10/12 00:00 [accepted]
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2011/02/05 06:00 [medline]
AID - S0014-4886(10)00389-4 [pii]
AID - 10.1016/j.expneurol.2010.10.004 [doi]
PST - ppublish
SO  - Exp Neurol. 2011 Jan;227(1):136-48. doi: 10.1016/j.expneurol.2010.10.004. Epub 
      2010 Oct 20.