PMID- 20969866
OWN - NLM
STAT- MEDLINE
DCOM- 20101220
LR  - 20161125
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 584
IP  - 22
DP  - 2010 Nov 19
TI  - Menin stimulates homology-directed DNA repair.
PG  - 4531-6
LID - 10.1016/j.febslet.2010.10.032 [doi]
AB  - Menin, the nuclear protein encoded by the Multiple Endocrine Neoplasia type 1 
      (MEN1) gene, acts as a tumor suppressor. It interacts with a large number of 
      proteins involved in chromatin modification, transcription, cell cycle checkpoint 
      and DNA repair, though its exact function is not clear. We report that in human 
      cells menin stimulates homology-directed (HD) DNA repair induced by the rare 
      endonuclease I-SceI and it accumulates with Chk1 at the site of the double strand 
      break. In addition, menin and Chk1 interact in vivo. Deletion of the first 228 
      amino acids of menin impairs the interaction with Chk1 and the ability to 
      stimulate HD repair, suggesting that the complex menin-Chk1 on the damaged 
      chromatin facilitates homologous recombination.
CI  - Copyright (c) 2010 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Gallo, A
AU  - Gallo A
AD  - Istituto di Endocrinologia ed Oncologia Sperimentale-Consiglio Nazionale delle 
      Ricerche and Dipartimento di Biologia e Patologia Cellulare e Molecolare, 
      Universita Federico II, 80131 Napoli, Italy. agallo@unina.it
FAU - Agnese, S
AU  - Agnese S
FAU - Esposito, I
AU  - Esposito I
FAU - Galgani, M
AU  - Galgani M
FAU - Avvedimento, V E
AU  - Avvedimento VE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101020
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (MEN1 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (CHEK1 protein, human)
RN  - EC 2.7.11.1 (Checkpoint Kinase 1)
SB  - IM
MH  - Checkpoint Kinase 1
MH  - DNA Breaks, Double-Stranded
MH  - DNA Repair/*genetics
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - Protein Kinases/metabolism
MH  - Proto-Oncogene Proteins/chemistry/genetics/*metabolism
MH  - *Recombination, Genetic
MH  - Sequence Deletion
EDAT- 2010/10/26 06:00
MHDA- 2010/12/21 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/08/05 00:00 [received]
PHST- 2010/09/30 00:00 [revised]
PHST- 2010/10/12 00:00 [accepted]
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2010/12/21 06:00 [medline]
AID - S0014-5793(10)00837-9 [pii]
AID - 10.1016/j.febslet.2010.10.032 [doi]
PST - ppublish
SO  - FEBS Lett. 2010 Nov 19;584(22):4531-6. doi: 10.1016/j.febslet.2010.10.032. Epub 
      2010 Oct 20.