PMID- 20969944
OWN - NLM
STAT- MEDLINE
DCOM- 20110526
LR  - 20220408
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 133
IP  - 2
DP  - 2011 Jan 27
TI  - Anti-inflammatory action of methanol extract of Carthamus tinctorius involves in 
      heme oxygenase-1 induction.
PG  - 524-30
LID - 10.1016/j.jep.2010.10.029 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: The methanol extracts of Carthamus tinctorius 
      (MEC) have long been used in traditional medicine as anti-inflammatory agent, 
      however, the molecular mechanism by which MEC shows anti-inflammatory action is 
      not investigated. AIM OF THE STUDY: Induction of heme oxygenase-1 (HO-1) by many 
      medicinal herbs has been reported excellent anti-inflammatory action. Thus, the 
      aim of the study is to explore whether anti-inflammatory action of MEC is related 
      with HO-1 induction in RAW 264.7 cells. MATERIALS AND METHODS: The present study 
      was designed to investigate as to MEC induces HO-1 expression so that it reduces 
      inflammation by suppression of inducible nitric oxide synthase (iNOS) and 
      cyclooxygenase-2 (COX-2) expression in cells activated with lipopolysaccharide 
      (LPS). RESULTS: Expression of HO-1 protein by MEC in macrophages was increased in 
      a concentration- and time-dependent manner. Treatment with MEC significantly 
      inhibited upregulation of both iNOS and COX-2 in LPS-activated macrophages and 
      consequently reduced production of NO and PGE(2), respectively. The reduced 
      expression of iNOS and COX-2 by MEC was reversed by siHO-1 RNA transfection. In 
      addition, NF-E2-related factor (Nrf2) was translocated from cytosol to nucleus by 
      MEC. The binding of NF-kappaB as well as NF-kappaB luciferase activity was also 
      significantly diminished by MEC. Finally, tumor necrosis factor (TNF)-alpha-mediated 
      VCAM-1 expression in endothelial cell was significantly inhibited by MEC. 
      CONCLUSIONS: The present results show that MEC induces HO-1 expression via Nrf2 
      translocation and inhibits NF-kappaB activity, which may be responsible for 
      anti-inflammatory action. Therefore, we propose that anti-inflammatory action of 
      MEC involves at least HO-1 induction.
CI  - Copyright A(c) 2010 Elsevier Ireland Ltd. All rights reserved.
FAU - Jun, Min Soo
AU  - Jun MS
AD  - Department of Pharmacology, School of Medicine, and Institute of Health Sciences, 
      Gyeongsang National University, 92 Chilam-dong, Jinju 660-751, Republic of Korea.
FAU - Ha, Yu Mi
AU  - Ha YM
FAU - Kim, Hee Sook
AU  - Kim HS
FAU - Jang, Hwa Jin
AU  - Jang HJ
FAU - Kim, Young Min
AU  - Kim YM
FAU - Lee, Young Soo
AU  - Lee YS
FAU - Kim, Hye Jung
AU  - Kim HJ
FAU - Seo, Han Geuk
AU  - Seo HG
FAU - Lee, Jae Heun
AU  - Lee JH
FAU - Lee, Seung Ho
AU  - Lee SH
FAU - Chang, Ki Churl
AU  - Chang KC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101020
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/isolation & purification/*pharmacology
MH  - Base Sequence
MH  - *Carthamus tinctorius/chemistry
MH  - Cell Line
MH  - Cyclooxygenase 2/metabolism
MH  - Dinoprostone/antagonists & inhibitors
MH  - Enzyme Induction/drug effects
MH  - Ethnopharmacology
MH  - Heme Oxygenase-1/antagonists & inhibitors/*biosynthesis/genetics
MH  - Macrophages/drug effects/metabolism
MH  - Medicine, Korean Traditional
MH  - Membrane Proteins/antagonists & inhibitors/*biosynthesis/genetics
MH  - Methanol
MH  - Mice
MH  - NF-E2-Related Factor 2/metabolism
MH  - NF-kappa B/antagonists & inhibitors
MH  - Nitric Oxide/antagonists & inhibitors
MH  - Nitric Oxide Synthase Type II/antagonists & inhibitors
MH  - Plant Extracts/isolation & purification/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - RNA, Small Interfering/genetics
MH  - Republic of Korea
MH  - Vascular Cell Adhesion Molecule-1/metabolism
EDAT- 2010/10/26 06:00
MHDA- 2011/05/27 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/06/11 00:00 [received]
PHST- 2010/10/05 00:00 [revised]
PHST- 2010/10/13 00:00 [accepted]
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2011/05/27 06:00 [medline]
AID - S0378-8741(10)00721-X [pii]
AID - 10.1016/j.jep.2010.10.029 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2011 Jan 27;133(2):524-30. doi: 10.1016/j.jep.2010.10.029. Epub 
      2010 Oct 20.