PMID- 20970116
OWN - NLM
STAT- MEDLINE
DCOM- 20110615
LR  - 20191210
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 3
DP  - 2011 Mar
TI  - Exposure of P. gingivalis to noradrenaline reduces bacterial growth and elevates 
      ArgX protease activity.
PG  - 244-50
LID - 10.1016/j.archoralbio.2010.09.014 [doi]
AB  - OBJECTIVE: Periodontitis, an infectious disease caused by periodontopathic 
      bacteria, including Porphyromonas gingivalis, is reported to be accelerated by 
      stress, under which noradrenaline levels are increased in the bloodstream. The 
      purpose of this study was to evaluate the effects of noradrenaline on P. 
      gingivalis. DESIGN: P. gingivalis was incubated in the presence of 25muM, 50muM, or 
      100muM adrenaline or noradrenaline at 37 degrees C for 12, 24 or 36h and growth was 
      evaluated by OD(660). Auto-inducer-2 (AI-2) was measured by luminescence of 
      Vibrio harveyi BB 170. Expression of P. gingivalis genes was evaluated using a 
      microarray and RT-PCR. Rgp activity of arg-gingipainA and B (Rgp) was measured 
      with a synthetic substrate. RESULTS: Growth of P. gingivalis FDC381 was inhibited 
      by noradrenaline at 24 and 36h. Growth inhibition by noradrenaline increased 
      dose-dependently. Inhibition of growth partially recovered with addition of 
      propranolol. AI-2 production from P. gingivalis showed a marked decrease with 
      addition of noradrenaline compared with peak production levels in the control 
      group. Microarray analysis revealed an increase in expression in 18 genes and a 
      decrease in expression in 2 genes. Amongst these genes, expression of the 
      protease arg-gingipainB (RgpB) gene, a major virulence factor of P. gingivalis, 
      was further analysed. Expression of rgpB showed a significant increase with 
      addition of noradrenaline, which was partially reduced by addition of 
      propranolol. Cell-associated Rgp activity also increased with addition of 
      noradrenaline. CONCLUSIONS: These results suggest that stressors influence the 
      expression of the virulence factors of P. gingivalis via noradrenaline.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Saito, Takayuki
AU  - Saito T
AD  - Oral Health Science Center, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, 
      Japan.
FAU - Inagaki, Satoru
AU  - Inagaki S
FAU - Sakurai, Kaoru
AU  - Sakurai K
FAU - Okuda, Katsuji
AU  - Okuda K
FAU - Ishihara, Kazuyuki
AU  - Ishihara K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101020
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Adrenergic alpha-Agonists)
RN  - 0 (Gingipain Cysteine Endopeptidases)
RN  - 0 (Virulence Factors)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Adhesins, Bacterial/genetics/*metabolism
MH  - Adrenergic alpha-Agonists/*pharmacology
MH  - Cysteine Endopeptidases/genetics/*metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Bacterial/drug effects
MH  - Gingipain Cysteine Endopeptidases
MH  - Norepinephrine/*pharmacology
MH  - Porphyromonas gingivalis/*drug effects/enzymology/growth & development
MH  - Stress, Physiological
MH  - Virulence Factors/genetics/*metabolism
EDAT- 2010/10/26 06:00
MHDA- 2011/06/16 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/04/16 00:00 [received]
PHST- 2010/09/16 00:00 [revised]
PHST- 2010/09/16 00:00 [accepted]
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2011/06/16 06:00 [medline]
AID - S0003-9969(10)00276-1 [pii]
AID - 10.1016/j.archoralbio.2010.09.014 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 Mar;56(3):244-50. doi: 10.1016/j.archoralbio.2010.09.014. 
      Epub 2010 Oct 20.