PMID- 20970676
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20101025
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 42
IP  - 8
DP  - 2010 Oct
TI  - Phenotype of dendritic cells generated from peripheral blood monocytes of 
      patients with ovarian cancer.
PG  - 3301-5
LID - 10.1016/j.transproceed.2010.07.037 [doi]
AB  - OBJECTIVES: The aim of our study was to generate dendritic cells (DCs) from 
      peripheral blood monocytes (PBMC) of patients suffering from ovarian cancer. 
      MATERIALS AND METHODS: Immature DCs were generated from PBMC cultured in RPMI 
      1640 medium with 2% human serum albumin (HSA), supplemented with recombinant 
      human (rh) granulocyte macrophage colony stimulating factor (GM-CSF) and rh 
      interleukin (IL)-4. After 5 days of culture, DC maturation was induced by the 
      addition of an ovarian cancer cell line (CAOV3) lysate and after 6 days of rh 
      tumor necrosis factor (TNF)-alpha for a further 2 days. The phenotype of the 
      generated cells was assessed by flow cytometry for the expressions of human 
      leukocyte antigen (HLA)-DR, costimulatory molecules (e.g., CD86, CD80), CD83, 
      CD1a, and CD14. PBMC cultured in 2% HSA without rhIL-4, rhGM-CSF, rh-TNF-alpha, or 
      tumor cell lysate formed the control group. RESULTS: The 2.41% (interquartile 
      range, 1.51%-3.52%) of CD45+/CD14+ cells in cultures with rhIL-4, rhGM-CSF, 
      rhTNF-alpha and tumor cell lysate was significantly lower than in the control group 
      (31.10%; interquartile range, 11.11%-64.06%). Cultures with rhIL-4, rhGM-CSF, 
      rhTNF-alpha, and tumor cell lysate showed a higher percentage (19.96%; interquartile 
      range, 9.30%-24.42%) of fully mature CD83+/CD1a-/HLA-DR+ DCs compared with 
      control culture (6.02%; interquartile range, 3.01%-7.37%). There was no 
      significant difference in the expression of the immature DC marker (CD1a) between 
      the cultures. The expression of co-stimulatory markers (CD80, CD86, HLA-DR) was 
      greater (24.29%; interquartile range, 18.68%-33.95%) on DCs from cultures with 
      rhIL-4, rhGM-CSF, TNF-alpha, and tumor cell lysate versus controls (4.93%; 
      interquartile range, 2.67%-9.09%). CONCLUSION: Our data demonstrated that 
      immature and mature DCs can be generated from adherent human PBMC from ovarian 
      cancer patients cultured with rhIL-4, rhGM-CSF, rhTNF-alpha, and tumor cell lysates.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Wertel, I
AU  - Wertel I
AD  - First Department of Gynaecology, Medical University, Lublin, Poland. 
      iwonawertel@wp.pl
FAU - Bednarek, W
AU  - Bednarek W
FAU - Stachowicz, N
AU  - Stachowicz N
FAU - Rogala, E
AU  - Rogala E
FAU - Nowicka, A
AU  - Nowicka A
FAU - Kotarski, J
AU  - Kotarski J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Culture Media)
SB  - IM
MH  - Cell Line, Tumor
MH  - Culture Media
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - *Immunophenotyping
MH  - Monocytes/*immunology
MH  - Ovarian Neoplasms/*immunology/pathology
EDAT- 2010/10/26 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - S0041-1345(10)01082-1 [pii]
AID - 10.1016/j.transproceed.2010.07.037 [doi]
PST - ppublish
SO  - Transplant Proc. 2010 Oct;42(8):3301-5. doi: 10.1016/j.transproceed.2010.07.037.