PMID- 20971061
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 402
IP  - 4
DP  - 2010 Nov 26
TI  - Immunomodulation of monocyte-derived dendritic cells through ligation of 
      tumor-produced mucins to Siglec-9.
PG  - 663-9
LID - 10.1016/j.bbrc.2010.10.079 [doi]
AB  - Dendritic cells (DCs) play an essential role in the induction and maintenance of 
      an effective immune response and express multiple siglecs. In the present study, 
      we investigated whether or not the ligation of tumor-produced mucins with 
      Siglec-9 expressed on immature DCs is related to escape from immunosurveillance 
      in the tumor-bearing state. Expression of Siglec-9 was up-regulated on the 
      development of monocytes into immature DCs and was decreased in mature DCs. 
      Binding of various mucins and artificial glycopolymers carrying poly (NeuAc alpha2,6 
      LacNAc) or poly (NeuAc alpha2,3 LacNAc) to Siglec-9 was demonstrated by means of a 
      plate assay. These mucins also bound to the surface of immature DCs. When 
      immature DCs were treated with LPS in the presence of these mucins or artificial 
      glycopolymers, the production of IL-12 was significantly reduced, but that of 
      IL-10 was not. Furthermore, IL-12 production was decreased to a similar level on 
      treatment with anti-Siglec-9 mAb. Mucins prepared from serum of cancer patients 
      actually could bind to Siglec-9. These results suggest that Siglec-9 expressed on 
      DCs is involved in immunoregulation through ligation with mucins in an epithelial 
      cancer patient.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Ohta, Mariko
AU  - Ohta M
AD  - Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo 
      University, Kita-ku, Kyoto, Japan.
FAU - Ishida, Akiko
AU  - Ishida A
FAU - Toda, Munetoyo
AU  - Toda M
FAU - Akita, Kaoru
AU  - Akita K
FAU - Inoue, Mizue
AU  - Inoue M
FAU - Yamashita, Keishi
AU  - Yamashita K
FAU - Watanabe, Masashi
AU  - Watanabe M
FAU - Murata, Takeomi
AU  - Murata T
FAU - Usui, Taichi
AU  - Usui T
FAU - Nakada, Hiroshi
AU  - Nakada H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101029
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Antigens, CD)
RN  - 0 (Lectins)
RN  - 0 (Mucins)
RN  - 0 (SIGLEC9 protein, human)
RN  - 0 (Sialic Acid Binding Immunoglobulin-like Lectins)
SB  - IM
MH  - Antigens, CD/*metabolism
MH  - Carcinoma/*immunology
MH  - Cell Line, Tumor
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Immunomodulation
MH  - Lectins/*metabolism
MH  - Monocytes/*immunology
MH  - Mucins/blood/*metabolism
MH  - Neoplasms/*immunology
MH  - Sialic Acid Binding Immunoglobulin-like Lectins
MH  - *Tumor Escape
EDAT- 2010/10/26 06:00
MHDA- 2011/01/14 06:00
CRDT- 2010/10/26 06:00
PHST- 2010/10/14 00:00 [received]
PHST- 2010/10/19 00:00 [accepted]
PHST- 2010/10/26 06:00 [entrez]
PHST- 2010/10/26 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
AID - S0006-291X(10)01957-1 [pii]
AID - 10.1016/j.bbrc.2010.10.079 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Nov 26;402(4):663-9. doi: 
      10.1016/j.bbrc.2010.10.079. Epub 2010 Oct 29.