PMID- 20972701 OWN - NLM STAT- MEDLINE DCOM- 20110805 LR - 20191210 IS - 1572-9729 (Electronic) IS - 0923-9820 (Linking) VI - 22 IP - 3 DP - 2011 Jun TI - Effects of temperature and initial pH on biohydrogen production from food-processing wastewater using anaerobic mixed cultures. PG - 551-63 LID - 10.1007/s10532-010-9427-z [doi] AB - This study attempted to determine the optimal temperature and initial cultivation pH by conducting a series of batch tests in stirred-tank bioreactor using fructose-producing wastewater as an organic substrate. The bioreactor temperature was controlled at 35-55 degrees C with an initial pH of 4-8. Hydrogen production efficiency was assessed using specific hydrogen production potential (SHPP) and the maximum specific hydrogen production rate (SHPR(m)). Experimental results indicated that temperature and initial pH markedly affected SHPP and SHPR(m), volatile fatty acids distribution as well as the ratio of butyrate/acetate (BHu/HAc). Two-fold higher SHPP and SHPR(m) were obtained at thermophilic condition (55 degrees C) than those at mesophilic condition (35 degrees C). The optimal initial pH was 6 for hydrogen production with peak values of SHPP of 166.8 ml-H(2)/g-COD and SHPR(m) of 26.7 ml-H(2)/g-VSS-h for fructose-processing wastewater. Molasses-processing wastewater had a higher SHPP (187.0 ml-H(2)/g-COD) and SHPR(m) (42.7 ml-H(2)/gVSS-h) than fructose-processing wastewater at pH 6. The DGGE profiles indicated that molasses-processing wastewater is a better substrate than fructose-processing wastewater for growth of hydrogen-producing bacteria due to the high staining intensity of bands. FAU - Lin, Yen-Hui AU - Lin YH AD - Department of Safety, Health and Environmental Engineering, Central Taiwan University of Science and Technology, Bei-tun District, Taichung, Taiwan. yhlin1@ctust.edu.tw FAU - Juan, Mu-Ling AU - Juan ML FAU - Hsien, Hsin-Jung AU - Hsien HJ LA - eng PT - Evaluation Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20101023 PL - Netherlands TA - Biodegradation JT - Biodegradation JID - 9100834 RN - 0 (Acetates) RN - 0 (Butyrates) RN - 0 (Industrial Waste) RN - 0 (Sewage) RN - 7YNJ3PO35Z (Hydrogen) SB - IM MH - Acetates/metabolism MH - Anaerobiosis MH - Bacteria/chemistry/*metabolism MH - Bioreactors/microbiology MH - Butyrates/metabolism MH - Food Handling MH - Hydrogen/*metabolism MH - Hydrogen-Ion Concentration MH - Industrial Microbiology/*methods MH - Industrial Waste/analysis MH - Molasses/analysis/microbiology MH - Sewage/*chemistry/microbiology MH - Temperature MH - Waste Disposal, Fluid/*methods EDAT- 2010/10/26 06:00 MHDA- 2011/08/06 06:00 CRDT- 2010/10/26 06:00 PHST- 2010/04/11 00:00 [received] PHST- 2010/10/09 00:00 [accepted] PHST- 2010/10/26 06:00 [entrez] PHST- 2010/10/26 06:00 [pubmed] PHST- 2011/08/06 06:00 [medline] AID - 10.1007/s10532-010-9427-z [doi] PST - ppublish SO - Biodegradation. 2011 Jun;22(3):551-63. doi: 10.1007/s10532-010-9427-z. Epub 2010 Oct 23.