PMID- 20973920
OWN - NLM
STAT- MEDLINE
DCOM- 20110616
LR  - 20211020
IS  - 1752-8062 (Electronic)
IS  - 1752-8054 (Print)
IS  - 1752-8054 (Linking)
VI  - 3
IP  - 5
DP  - 2010 Oct
TI  - Altered immune phenotype in peripheral blood cells of patients with 
      scleroderma-associated pulmonary hypertension.
PG  - 210-8
LID - 10.1111/j.1752-8062.2010.00218.x [doi]
AB  - Pulmonary arterial hypertension is a common and fatal complication of scleroderma 
      that may involve inflammatory and autoimmune mechanisms. Alterations in the gene 
      expression of peripheral blood mononuclear cells have been previously described 
      in patients with pulmonary arterial hypertension. Our goal is to identify 
      differentially expressed genes in peripheral blood mononuclear cells in 
      scleroderma patients with and without pulmonary hypertension as biomarkers of 
      disease. Gene expression analysis was performed on a Microarray Cohort of 
      scleroderma patients with (n = 10) and without (n = 10) pulmonary hypertension. 
      Differentially expressed genes were confirmed in the Microarray Cohort and 
      validated in a Validation Cohort of scleroderma patients with (n = 15) and 
      without (n = 19) pulmonary hypertension by RT-qPCR. We identified inflammatory 
      and immune-related genes including interleukin-7 receptor (IL-7R) and chemokine 
      receptor 7 as differentially expressed in patients with scleroderma-associated 
      pulmonary hypertension. Flow cytometry confirmed decreased expression of IL-7R on 
      circulating CD4+ T-cells from scleroderma patients with pulmonary hypertension. 
      Differences exist in the expression of inflammatory and immune-related genes in 
      peripheral blood cells from patients with scleroderma-related pulmonary 
      hypertension compared to those with normal pulmonary artery pressures. These 
      findings may have implications as biomarkers to screen at-risk populations for 
      early diagnosis and provide insight into mechanisms of scleroderma-related 
      pulmonary hypertension.
CI  - (c) 2010 Wiley Periodicals, Inc.
FAU - Risbano, Michael G
AU  - Risbano MG
AD  - Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado 
      Denver, Aurora, USA. onabsir@gmail.com
FAU - Meadows, Christina A
AU  - Meadows CA
FAU - Coldren, Christopher D
AU  - Coldren CD
FAU - Jenkins, Tiffany J
AU  - Jenkins TJ
FAU - Edwards, Michael G
AU  - Edwards MG
FAU - Collier, David
AU  - Collier D
FAU - Huber, Wendy
AU  - Huber W
FAU - Mack, Douglas G
AU  - Mack DG
FAU - Fontenot, Andrew P
AU  - Fontenot AP
FAU - Geraci, Mark W
AU  - Geraci MW
FAU - Bull, Todd M
AU  - Bull TM
LA  - eng
GR  - K08 HL072858/HL/NHLBI NIH HHS/United States
GR  - L30 HL097867/HL/NHLBI NIH HHS/United States
GR  - K08 HLO72858-01A2/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Transl Sci
JT  - Clinical and translational science
JID - 101474067
RN  - 0 (Receptors, Interleukin-7)
SB  - IM
EIN - Clin Transl Sci. 2010 Dec;3(6):340. Hunt, James [added]
CIN - Clin Transl Sci. 2011 Feb;4(1):72. PMID: 21348960
MH  - Blood Cells/*immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cluster Analysis
MH  - Cohort Studies
MH  - Demography
MH  - Familial Primary Pulmonary Hypertension
MH  - Female
MH  - Flow Cytometry
MH  - Hemodynamics
MH  - Humans
MH  - Hypertension, Pulmonary/blood/etiology/genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Receptors, Interleukin-7/immunology
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Scleroderma, Systemic/*blood/*complications/genetics/immunology
PMC - PMC2966033
MID - NIHMS224993
EDAT- 2010/10/27 06:00
MHDA- 2011/06/17 06:00
PMCR- 2010/10/01
CRDT- 2010/10/27 06:00
PHST- 2010/10/27 06:00 [entrez]
PHST- 2010/10/27 06:00 [pubmed]
PHST- 2011/06/17 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - CTS218 [pii]
AID - 10.1111/j.1752-8062.2010.00218.x [doi]
PST - ppublish
SO  - Clin Transl Sci. 2010 Oct;3(5):210-8. doi: 10.1111/j.1752-8062.2010.00218.x.