PMID- 20974144
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20220129
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Linking)
VI  - 41
IP  - 2
DP  - 2011 Feb
TI  - Ascaris lumbricoides pseudocoelomic body fluid induces a partially activated 
      dendritic cell phenotype with Th2 promoting ability in vivo.
PG  - 255-61
LID - 10.1016/j.ijpara.2010.09.007 [doi]
AB  - Dendritic cells (DCs) matured with helminth-derived molecules that promote Th2 
      immune responses do not follow conventional definitions of DC maturation 
      processes. While a number of models of DC maturation by Th2 stimuli are 
      postulated, further studies are required if we are to clearly define DC 
      maturation processes that lead to Th2 immune responses. In this study, we examine 
      the interaction of Th2-inducing molecules from the parasitic helminth Ascaris 
      lumbricoides with the maturation processes and function of DCs. Here we show that 
      murine bone marrow-derived DCs are partially matured by A. lumbricoides 
      pseudocoelomic body fluid (ABF) as characterised by the production of IL-6, 
      IL-12p40 and macrophage inflammatory protein 2 (MIP-2) but no enhanced expression 
      of cluster of differentiation (CD)-14, T-cell co-stimulatory markers CD80, CD86, 
      CD40, OX40L and major histocompatibility complex class II was observed. Despite 
      these phenotypic characteristics, ABF-stimulated DCs displayed the functional 
      hallmarks of fully matured cells, enhancing DC phagocytosis and promoting 
      Th2-type responses in skin-draining lymph node cells in vivo. ABF activated 
      Th2-associated extracellular signal-regulated kinase-1 and nuclear factor-kB 
      intracellular signalling pathways independently of toll-like receptor 4. Taken 
      together, we believe this is the first paper to demonstrate A. lumbricoides 
      murine DC-Th cell-driven responses shedding further light on DC maturation 
      processes by helminth antigens.
CI  - Copyright (c) 2010 Australian Society for Parasitology Inc. Published by Elsevier 
      Ltd. All rights reserved.
FAU - Dowling, David J
AU  - Dowling DJ
AD  - Parasite Immune Modulation Group, School of Nursing, Faculty of Science and 
      Health, Dublin City University, Glasnevin, Dublin 9, Ireland.
FAU - Noone, Cariosa M
AU  - Noone CM
FAU - Adams, Paul N
AU  - Adams PN
FAU - Vukman, Krisztina V
AU  - Vukman KV
FAU - Molloy, Sile F
AU  - Molloy SF
FAU - Forde, Jessica
AU  - Forde J
FAU - Asaolu, Samuel
AU  - Asaolu S
FAU - O'Neill, Sandra M
AU  - O'Neill SM
LA  - eng
GR  - BB/C503638/2/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101023
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Ascaris lumbricoides/*immunology
MH  - Body Fluids/*immunology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Lymph Nodes/immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Phagocytosis
MH  - Th2 Cells/*immunology
EDAT- 2010/10/27 06:00
MHDA- 2011/05/04 06:00
CRDT- 2010/10/27 06:00
PHST- 2010/05/26 00:00 [received]
PHST- 2010/09/14 00:00 [revised]
PHST- 2010/09/15 00:00 [accepted]
PHST- 2010/10/27 06:00 [entrez]
PHST- 2010/10/27 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
AID - S0020-7519(10)00322-X [pii]
AID - 10.1016/j.ijpara.2010.09.007 [doi]
PST - ppublish
SO  - Int J Parasitol. 2011 Feb;41(2):255-61. doi: 10.1016/j.ijpara.2010.09.007. Epub 
      2010 Oct 23.