PMID- 20976044
OWN - NLM
STAT- MEDLINE
DCOM- 20110224
LR  - 20211020
IS  - 1545-7885 (Electronic)
IS  - 1544-9173 (Print)
IS  - 1544-9173 (Linking)
VI  - 8
IP  - 10
DP  - 2010 Oct 19
TI  - Frequent and efficient use of the sister chromatid for DNA double-strand break 
      repair during budding yeast meiosis.
PG  - e1000520
LID - 10.1371/journal.pbio.1000520 [doi]
LID - e1000520
AB  - Recombination between homologous chromosomes of different parental origin 
      (homologs) is necessary for their accurate segregation during meiosis. It has 
      been suggested that meiotic inter-homolog recombination is promoted by a barrier 
      to inter-sister-chromatid recombination, imposed by meiosis-specific components 
      of the chromosome axis. Consistent with this, measures of Holliday 
      junction-containing recombination intermediates (joint molecules [JMs]) show a 
      strong bias towards inter-homolog and against inter-sister JMs. However, 
      recombination between sister chromatids also has an important role in meiosis. 
      The genomes of diploid organisms in natural populations are highly polymorphic 
      for insertions and deletions, and meiotic double-strand breaks (DSBs) that form 
      within such polymorphic regions must be repaired by inter-sister recombination. 
      Efforts to study inter-sister recombination during meiosis, in particular to 
      determine recombination frequencies and mechanisms, have been constrained by the 
      inability to monitor the products of inter-sister recombination. We present here 
      molecular-level studies of inter-sister recombination during budding yeast 
      meiosis. We examined events initiated by DSBs in regions that lack corresponding 
      sequences on the homolog, and show that these DSBs are efficiently repaired by 
      inter-sister recombination. This occurs with the same timing as inter-homolog 
      recombination, but with reduced (2- to 3-fold) yields of JMs. Loss of the 
      meiotic-chromosome-axis-associated kinase Mek1 accelerates inter-sister DSB 
      repair and markedly increases inter-sister JM frequencies. Furthermore, 
      inter-sister JMs formed in mek1Delta mutants are preferentially lost, while 
      inter-homolog JMs are maintained. These findings indicate that inter-sister 
      recombination occurs frequently during budding yeast meiosis, with the 
      possibility that up to one-third of all recombination events occur between sister 
      chromatids. We suggest that a Mek1-dependent reduction in the rate of 
      inter-sister repair, combined with the destabilization of inter-sister JMs, 
      promotes inter-homolog recombination while retaining the capacity for 
      inter-sister recombination when inter-homolog recombination is not possible.
FAU - Goldfarb, Tamara
AU  - Goldfarb T
AD  - Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, 
      National Cancer Institute, Bethesda, Maryland, United States of America.
FAU - Lichten, Michael
AU  - Lichten M
LA  - eng
GR  - Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20101019
PL  - United States
TA  - PLoS Biol
JT  - PLoS biology
JID - 101183755
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MSH4 protein, S cerevisiae)
RN  - 0 (Saccharomyces cerevisiae Proteins)
SB  - IM
CIN - PLoS Biol. 8:e1000519.
MH  - Chromatids/*genetics
MH  - *DNA Breaks, Double-Stranded
MH  - *DNA Repair
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Meiosis/*genetics
MH  - Saccharomyces cerevisiae/*genetics/metabolism
MH  - Saccharomyces cerevisiae Proteins/genetics/metabolism
PMC - PMC2957403
COIS- The authors have declared that no competing interests exist.
EDAT- 2010/10/27 06:00
MHDA- 2011/02/25 06:00
PMCR- 2010/10/19
CRDT- 2010/10/27 06:00
PHST- 2010/04/15 00:00 [received]
PHST- 2010/09/02 00:00 [accepted]
PHST- 2010/10/27 06:00 [entrez]
PHST- 2010/10/27 06:00 [pubmed]
PHST- 2011/02/25 06:00 [medline]
PHST- 2010/10/19 00:00 [pmc-release]
AID - 10-PLBI-RA-7044R2 [pii]
AID - 10.1371/journal.pbio.1000520 [doi]
PST - epublish
SO  - PLoS Biol. 2010 Oct 19;8(10):e1000520. doi: 10.1371/journal.pbio.1000520.