PMID- 20977484
OWN - NLM
STAT- MEDLINE
DCOM- 20110613
LR  - 20211020
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Print)
IS  - 0041-1132 (Linking)
VI  - 51
IP  - 4
DP  - 2011 Apr
TI  - The frequency and specificity of human neutrophil antigen antibodies in a blood 
      donor population.
PG  - 820-7
LID - 10.1111/j.1537-2995.2010.02913.x [doi]
AB  - BACKGROUND: Transfusion-related acute lung injury (TRALI) has been associated 
      with both human leukocyte antigen (HLA) and human neutrophil antigen (HNA) 
      antibodies. HNA antibody frequency, specificity, and demographic associations 
      have not been well defined in the blood donor population. STUDY DESIGN AND 
      METHODS: A subset of 1171 donors (388 nontransfused males, 390 HLA 
      antibody-negative females with three or more pregnancies, and 393 HLA 
      antibody-positive females with three or more pregnancies) from a larger Leukocyte 
      Antibody Prevalence Study was tested for immunoglobulin (Ig)G and IgM HNA 
      antibody using a granulocyte immunofluorescence flow cytometry assay. Additional 
      testing on selected samples included monoclonal antibody immobilization of 
      granulocyte antigen-flow cytometry and granulocyte genotyping. RESULTS: Eight 
      samples were HNA antibody positive (prevalence, 0.7%; 95% confidence interval 
      [CI], 0.3%-1.3%]). Three HNA antibodies (one IgG and two IgM) were found in 
      nontransfused males (prevalence, 0.8%; 95% CI, 0.2%-2.2%); all were panreactive 
      or nonspecific. One HLA antibody-negative previously pregnant female had an IgG 
      HNA antibody with HNA-1a specificity (prevalence, 0.3%; 95% CI, 0.01%-1.4%). Four 
      HLA antibody-positive previously pregnant females demonstrated HNA antibodies, 
      three IgG and one IgM (prevalence, 1%; 95% CI, 0.3%-2.6%). Two of these were 
      HNA-1a specific, one HNA-4a specific, and one nonspecific. CONCLUSIONS: HNA 
      antibodies occur with low frequency in the donor population and are present in 
      both male and female donors. Despite the implementation of TRALI reduction 
      strategies, HNA antibodies are still present in donor blood products. Although 
      our data do not create a case for urgent implementation of donor HNA antibody 
      testing, future new developments for high-throughput HNA antibody screening, 
      including for HNA-3a, may warrant reconsideration.
CI  - (c) 2010 American Association of Blood Banks.
FAU - Gottschall, Jerome L
AU  - Gottschall JL
AD  - BloodCenter ofWisconsin, Milwaukee, Wisconsin 53233, USA. 
      jerry.gottschall@bcw.edu
FAU - Triulzi, Darrell J
AU  - Triulzi DJ
FAU - Curtis, Brian
AU  - Curtis B
FAU - Kakaiya, Ram M
AU  - Kakaiya RM
FAU - Busch, Michael P
AU  - Busch MP
FAU - Norris, Philip J
AU  - Norris PJ
FAU - Glynn, Simone A
AU  - Glynn SA
FAU - Carrick, Danielle
AU  - Carrick D
FAU - Wright, David J
AU  - Wright DJ
FAU - Kleinman, Steve
AU  - Kleinman S
CN  - NHLBI Retrovirus Epidemiology Donor Study II
LA  - eng
GR  - N01-HB-47172/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47171/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47170/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47175/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47174/HB/NHLBI NIH HHS/United States
GR  - N01-HB-47169/HB/NHLBI NIH HHS/United States
GR  - HHSN268200517181C/HL/NHLBI NIH HHS/United States
GR  - N01-HB-47168/HB/NHLBI NIH HHS/United States
GR  - N01-HB-57181/HB/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, N.I.H., Extramural
DEP - 20101026
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (Autoantibodies)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Acute Lung Injury/*etiology/immunology
MH  - Antibodies, Antineutrophil Cytoplasmic/immunology
MH  - Autoantibodies/*immunology
MH  - *Blood Donors
MH  - Cross-Sectional Studies
MH  - Female
MH  - Flow Cytometry
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - *Transfusion Reaction
PMC - PMC3583295
MID - NIHMS241292
EDAT- 2010/10/28 06:00
MHDA- 2011/06/15 06:00
PMCR- 2013/02/27
CRDT- 2010/10/28 06:00
PHST- 2010/10/28 06:00 [entrez]
PHST- 2010/10/28 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
PHST- 2013/02/27 00:00 [pmc-release]
AID - 10.1111/j.1537-2995.2010.02913.x [doi]
PST - ppublish
SO  - Transfusion. 2011 Apr;51(4):820-7. doi: 10.1111/j.1537-2995.2010.02913.x. Epub 
      2010 Oct 26.