PMID- 20979138
OWN - NLM
STAT- MEDLINE
DCOM- 20110208
LR  - 20220410
IS  - 1549-4918 (Electronic)
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 28
IP  - 10
DP  - 2010 Oct
TI  - Allogeneic periodontal ligament stem cell therapy for periodontitis in swine.
PG  - 1829-38
LID - 10.1002/stem.512 [doi]
AB  - Periodontitis is one of the most widespread infectious diseases in humans. It is 
      the main cause of tooth loss and associated with a number of systemic diseases. 
      Until now, there is no appropriate method for functional periodontal tissue 
      regeneration. Here, we establish a novel approach of using allogeneic periodontal 
      ligament stem cells (PDLSCs) sheet to curing periodontitis in a miniature pig 
      periodontitis model. Significant periodontal tissue regeneration was achieved in 
      both the autologous and the allogeneic PDLSCs transplantation group at 12 weeks 
      post-PDLSCs transplantation. Based on clinical assessments, computed tomography 
      (CT) scanning, and histological examination, there was no marked difference 
      between the autologous and allogeneic PDLSCs transplantation groups. In addition, 
      lack of immunological rejections in the animals that received the allogeneic 
      PDLSCs transplantation was observed. Interestingly, we found that human PDLSCs 
      fail to express human leukocyte antigen (HLA)-II DR and costimulatory molecules. 
      PDLSCs were not able to elicit T-cell proliferation and inhibit T-cell 
      proliferation when stimulated with mismatched major histocompatibility complex 
      molecules. Furthermore, we found that prostaglandin E2 (PGE2) plays a crucial 
      role in PDLSCs-mediated immunomodulation and periodontal tissue regeneration in 
      vitro and in vivo. Our study demonstrated that PDLSCs possess low immunogenicity 
      and marked immunosuppression via PGE2-induced T-cell anergy. We developed a 
      standard technological procedure of using allogeneic PDLSCs to cure periodontitis 
      in swine.
FAU - Ding, Gang
AU  - Ding G
AD  - Molecular Laboratory for Gene Therapy and Tooth Regeneration, Capital Medical 
      University School of Stomatology, Beijing, People's Republic of China.
FAU - Liu, Yi
AU  - Liu Y
FAU - Wang, Wei
AU  - Wang W
FAU - Wei, Fulan
AU  - Wei F
FAU - Liu, Dayong
AU  - Liu D
FAU - Fan, Zhipeng
AU  - Fan Z
FAU - An, Yunqing
AU  - An Y
FAU - Zhang, Chunmei
AU  - Zhang C
FAU - Wang, Songlin
AU  - Wang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Flow Cytometry
MH  - Humans
MH  - Periodontal Ligament/*cytology
MH  - Periodontitis/*therapy
MH  - Polymerase Chain Reaction
MH  - Stem Cell Transplantation/*methods
MH  - Stem Cells/cytology/metabolism
MH  - Swine
MH  - Tissue Engineering/methods
PMC - PMC2996858
EDAT- 2010/10/28 06:00
MHDA- 2011/02/09 06:00
PMCR- 2010/12/03
CRDT- 2010/10/28 06:00
PHST- 2010/10/28 06:00 [entrez]
PHST- 2010/10/28 06:00 [pubmed]
PHST- 2011/02/09 06:00 [medline]
PHST- 2010/12/03 00:00 [pmc-release]
AID - 10.1002/stem.512 [doi]
PST - ppublish
SO  - Stem Cells. 2010 Oct;28(10):1829-38. doi: 10.1002/stem.512.