PMID- 20979787
OWN - NLM
STAT- MEDLINE
DCOM- 20110111
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 49
IP  - 8
DP  - 2010 Aug
TI  - [The relationship of methylenetetrahydrofolate reductase G1793A gene 
      polymorphism, hyperhomocysteinaemia and ulcerative colitis].
PG  - 675-9
AB  - OBJECTIVES: The present study aimed to investigate the associations between 
      genetic polymorphism of methylenetetrahydrofolate reductase (MTHFR) G1793A, 
      plasma homocysteine (Hcy) levels, vitamin status and ulcerative colitis (UC) in a 
      cohort of patients in Hubei Han nationality. METHODS: Two hundred and ninety-nine 
      UC patients and 764 age- and sex-matched healthy controls were recruited in this 
      study. Polymorphism of MTHFR G1793A was examined using a PCR-RELP method. Plasma 
      levels of Hcy, folate and vitamin B12 were determined by enzymatic cycling assay 
      and corpuscle immune chemiluminescence assay, respectively. RESULTS: Both variant 
      allele and genotype frequencies in MTHFR G1793A gene were significantly higher in 
      the UC patients compared to the controls (22.24% vs 14.20%, P < 0.001; 42.81% vs 
      26.97%, P<0.001, respectively). Plasma Hcy levels were increased in UC patients 
      compared to the controls [(20.67 +/- 6.42) mmol/L vs (13.21+/-5.11) mmol/L, P<0.001] 
      while folate and vitamin B12 concentrations were significantly decreased 
      [(11.37+/-6.34) nmol/L vs (14.89+/-7.21) nmol/L, P<0.001; (324.15+/-127.53) pmol/L vs 
      (421.54+/-128.45) pmol/L, P<0.001, respectively]. Furthermore, 
      hyperhomocysteinaemia (HHcy) and folate deficiency were also more prevalent in 
      the UC patients (32.44% vs 25.78%, P=0.029; 23.41% vs 17.01%, P=0.016, 
      respectively). CONCLUSIONS: Genetic polymorphism of MTHFR G1793A was strongly 
      associated with UC. HHcy, folate deficiency and low vitamin B12 concentration 
      were common phenomena in the UC patients of Hubei Han nationality. Our findings 
      demonstrate that the genes related to Hcy metabolism may play an important role 
      in the pathogenesis of UC.
FAU - Jiang, Yi
AU  - Jiang Y
AD  - Department of Gastroenterology, Wuhan University Zhongnan Hospital, Wuhan 430071, 
      China.
FAU - Zhao, Jie
AU  - Zhao J
FAU - Xu, Chang-long
AU  - Xu CL
FAU - Cao, Shu-guang
AU  - Cao SG
FAU - Lin, Li-miao
AU  - Lin LM
FAU - Lei, Yuan
AU  - Lei Y
FAU - Huang, Sha
AU  - Huang S
FAU - Wang, Chang-gao
AU  - Wang CG
FAU - Xia, Bing
AU  - Xia B
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 935E97BOY8 (Folic Acid)
RN  - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2))
RN  - P6YC3EG204 (Vitamin B 12)
SB  - IM
MH  - Adult
MH  - Case-Control Studies
MH  - Colitis, Ulcerative/*blood/*genetics
MH  - Female
MH  - Folic Acid/blood
MH  - Folic Acid Deficiency/complications
MH  - Homocysteine/blood
MH  - Humans
MH  - Hyperhomocysteinemia/etiology/*genetics/metabolism
MH  - Male
MH  - Methylenetetrahydrofolate Reductase (NADPH2)/*genetics
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Vitamin B 12/blood
EDAT- 2010/10/29 06:00
MHDA- 2011/01/12 06:00
CRDT- 2010/10/29 06:00
PHST- 2010/10/29 06:00 [entrez]
PHST- 2010/10/29 06:00 [pubmed]
PHST- 2011/01/12 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2010 Aug;49(8):675-9.