PMID- 20979875
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20141120
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 90
IP  - 24
DP  - 2010 Jun 22
TI  - [Automated silver-enhanced in situ hybridization detection assay for human 
      epidermal growth factor receptor 2 gene status determination in breast cancer].
PG  - 1674-7
AB  - OBJECTIVE: To evaluate the concordance of human epidermal growth factor receptor 
      2 (HER2) gene amplification in invasive breast cancer by the techniques of 
      fluorescence in situ hybridization (FISH) and silver-enhanced in situ 
      hybridization (SISH). METHODS: A prospective study of 63 invasive breast cancer 
      specimens excised between May 2009 and November 2009 was conducted by automated 
      immunohistochemistry (IHC) staining and bright field automated SISH. HER2 gene 
      status was also examined by FISH in 43 cases. An evaluation was performed 
      according to the ASCO/CAP guidelines (2007). RESULTS: Among 63 breast invasive 
      ductal carcinoma tested by automated SISH, 61 cases were successfully evaluated. 
      Gene amplification was detected in 97% (29/30) HER2 protein expression positive 
      (3+) cases and in 18% (2/11) equivocal (2+) cases. Two of 11 (18%) HER2 
      expression 2+ cases were equivocal for gene amplification. In 43 cases examined 
      by FISH, HER2 gene amplification was detected in 95% (20/21) HER2 expression 
      positive (3+) cases and in one equivocal (2+) cases. Three of 10 (30%) expression 
      2+ cases were equivocal for gene amplification. No gene amplification was 
      detected in expression negative (1 +/0) cases by either SISH or FISH (0/20 and 
      0/12 respectively). The results of SISH and FISH were identical in 42 cases 
      examined by the above two techniques, including 21 HER2 gene amplification 
      positive, 2 equivocal and 19 negative cases. One case was FISH equivocal and SISH 
      negative. And the overall concordance between FISH and SISH was 98%. CONCLUSIONS: 
      There is high concordance rate between SISH and FISH results for assessing the 
      HER2 gene amplification status of excised breast cancer specimens. However, SISH 
      has more advantages and it is particularly suited for routine application in 
      surgical pathology.
FAU - Ying, Jian-ming
AU  - Ying JM
AD  - Department of Pathology, Cancer Hospital, Peking Union Medical College and 
      Chinese Academy of Medical Sciences, Beijing.
FAU - Guo, Lei
AU  - Guo L
FAU - Liu, Xiu-yun
AU  - Liu XY
FAU - Qiu, Tian
AU  - Qiu T
FAU - Lu, Ning
AU  - Lu N
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Silver Compounds)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma, Ductal, Breast/*genetics/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Middle Aged
MH  - Prospective Studies
MH  - Receptor, ErbB-2/*genetics
MH  - Silver Compounds
EDAT- 2010/10/29 06:00
MHDA- 2011/02/04 06:00
CRDT- 2010/10/29 06:00
PHST- 2010/10/29 06:00 [entrez]
PHST- 2010/10/29 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2010 Jun 22;90(24):1674-7.