PMID- 20980482
OWN - NLM
STAT- MEDLINE
DCOM- 20110307
LR  - 20211216
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 17
IP  - 12
DP  - 2010 Dec
TI  - Escherichia coli K88ac fimbriae expressing heat-labile and heat-stable (STa) 
      toxin epitopes elicit antibodies that neutralize cholera toxin and STa toxin and 
      inhibit adherence of K88ac fimbrial E. coli.
PG  - 1859-67
LID - 10.1128/CVI.00251-10 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) strains are a major cause of diarrheal 
      disease in humans and animals. Bacterial adhesins and heat-labile (LT) and 
      heat-stable (ST) enterotoxins are the virulence determinants in ETEC diarrhea. It 
      is believed that vaccines inducing anti-adhesin immunity to inhibit bacterial 
      adherence and anti-toxin immunity to eliminate toxin activity would provide 
      broad-spectrum protection against ETEC. In this study, an ETEC fimbrial adhesin 
      was used as a platform to express LT and STa for adhesin-toxin fusion antigens to 
      induce anti-toxin and anti-adhesin immunity. An epitope from the B subunit of LT 
      toxin (LTP1, (8)LCSEYRNTQIYTIN(21)) and an STa toxoid epitope 
      ((5)CCELCCNPQCAGCY(18)) were embedded in the FaeG major subunit of E. coli K88ac 
      fimbriae. Constructed K88ac-toxin chimeric fimbriae were harvested and used for 
      rabbit immunization. Immunized rabbits developed anti-K88ac, anti-LT, and 
      anti-STa antibodies. Moreover, induced antibodies not only inhibited adherence of 
      K88ac fimbrial E. coli to porcine small intestinal enterocytes but also 
      neutralized cholera toxin and STa toxin. Data from this study demonstrated that 
      K88ac fimbriae expressing LT and STa epitope antigens elicited neutralizing 
      anti-toxin antibodies and anti-adhesin antibodies and suggested that E. coli 
      fimbriae could serve as a platform for the development of broad-spectrum vaccines 
      against ETEC.
FAU - Zhang, Chengxian
AU  - Zhang C
AD  - Veterinary and Biomedical Sciences Department, The Center for Infectious Disease 
      Research and Vaccinology, South Dakota State University, Box 2157, North Campus 
      Drive, Brookings, SD 57006, USA.
FAU - Zhang, Weiping
AU  - Zhang W
LA  - eng
GR  - R03 AI068766/AI/NIAID NIH HHS/United States
GR  - AI068766/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20101027
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Epitopes)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Escherichia coli Vaccines)
RN  - 0 (K88 antigen, E coli)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (heat stable toxin (E coli))
RN  - 147680-16-8 (Fimbriae Proteins)
RN  - 9012-63-9 (Cholera Toxin)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Antibodies, Bacterial/blood
MH  - Antibodies, Neutralizing/*blood
MH  - Antigens, Bacterial/genetics/*immunology
MH  - Bacterial Adhesion/*immunology
MH  - Bacterial Toxins/antagonists & inhibitors/genetics/*immunology
MH  - Cells, Cultured
MH  - Cholera Toxin/*antagonists & inhibitors/immunology
MH  - Enterotoxigenic Escherichia coli/immunology/pathogenicity
MH  - Enterotoxins/antagonists & inhibitors/genetics/*immunology
MH  - Epithelial Cells/microbiology
MH  - Epitopes/genetics/immunology
MH  - Escherichia coli Infections/prevention & control
MH  - Escherichia coli Proteins/genetics/*immunology
MH  - Escherichia coli Vaccines/genetics/*immunology
MH  - Fimbriae Proteins/genetics/*immunology
MH  - Rabbits
MH  - Swine
MH  - Vaccines, Synthetic/genetics/immunology
PMC - PMC3008177
EDAT- 2010/10/29 06:00
MHDA- 2011/03/08 06:00
PMCR- 2011/06/01
CRDT- 2010/10/29 06:00
PHST- 2010/10/29 06:00 [entrez]
PHST- 2010/10/29 06:00 [pubmed]
PHST- 2011/03/08 06:00 [medline]
PHST- 2011/06/01 00:00 [pmc-release]
AID - CVI.00251-10 [pii]
AID - 0251-10 [pii]
AID - 10.1128/CVI.00251-10 [doi]
PST - ppublish
SO  - Clin Vaccine Immunol. 2010 Dec;17(12):1859-67. doi: 10.1128/CVI.00251-10. Epub 
      2010 Oct 27.