PMID- 2101928
OWN - NLM
STAT- MEDLINE
DCOM- 19910918
LR  - 20191022
IS  - 0893-5785 (Print)
IS  - 0893-5785 (Linking)
VI  - 3
IP  - 6
DP  - 1990 Dec
TI  - Further evidence for synthesis of screening pigment granules involved in the 
      photosensory membrane turnover of the crayfish photoreceptor.
PG  - 279-89
AB  - Photosensory membrane degradation in crayfish occurs at first in multi-vesicular 
      bodies (MVBs) and then, with the aid of lysosomal enzymes, in lysosome related 
      lamellar bodies. In organ culture experiments with the isolated crayfish retina 
      (Orconectes limosus) small screening pigment-like granules became visible under 
      the electron microscope in such lamellar bodies and suggested a possible relation 
      of photosensory membrane degradation and screening pigment granule synthesis. 
      Chloroquine, an inhibitor of lysosomal activity, when added to the culture medium 
      reduced the appearance of screening pigment-like granules in lamellar bodies, but 
      led to the appearance of these granules in mature MVB's, indicating the 
      involvement of lysosomal enzymes in the formation of pigmented lamellar bodies. 
      In a second set of experiments the effect of bright light on the screening 
      pigment granule ultrastructure of crayfish phoreceptors was investigated. It was 
      found that after bright light exposure large numbers of little screening pigment 
      granules (0.15-0.3 microns) were located between or close to rhabdomeral 
      microvilli that were not at these sites in crayfish kept under natural light. 
      MVB's were also reduced in size, and among the little screening pigmentary 
      organelles granules of different electron density and morphology appeared. 
      Additionally, vesicle flux to little screening pigment granules was detected. The 
      screening pigment granules of the little type did not seem to be transported 
      close to or between the microvilli, but appeared to be synthesized at these sites 
      within little MVBs.
FAU - Schraermeyer, U
AU  - Schraermeyer U
AD  - Institut fur Biologie II (Zoologie), RWTH Aachen, FRG.
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Pigment Cell Res
JT  - Pigment cell research
JID - 8800247
RN  - 0 (Pigments, Biological)
RN  - 886U3H6UFF (Chloroquine)
SB  - IM
MH  - Animals
MH  - Astacoidea/*metabolism
MH  - Chloroquine/pharmacology
MH  - Chromatophores/*metabolism/radiation effects/ultrastructure
MH  - Intracellular Membranes/metabolism
MH  - Light
MH  - Lysosomes/metabolism/ultrastructure
MH  - Microscopy, Electron
MH  - Microvilli/metabolism/ultrastructure
MH  - Organ Culture Techniques
MH  - Photoreceptor Cells/*metabolism/radiation effects
MH  - Pigments, Biological/*biosynthesis
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
AID - 10.1111/j.1600-0749.1990.tb00299.x [doi]
PST - ppublish
SO  - Pigment Cell Res. 1990 Dec;3(6):279-89. doi: 10.1111/j.1600-0749.1990.tb00299.x.