PMID- 21029097
OWN - NLM
STAT- MEDLINE
DCOM- 20101201
LR  - 20161125
IS  - 1748-5827 (Electronic)
IS  - 0022-4510 (Linking)
VI  - 51
IP  - 10
DP  - 2010 Oct
TI  - Haemoplasma infection is not a common cause of canine immune-mediated haemolytic 
      anaemia in the UK.
PG  - 534-9
LID - 10.1111/j.1748-5827.2010.00987.x [doi]
AB  - OBJECTIVE: The aim of this study was to investigate whether the two canine 
      haemoplasma species, Mycoplasma haemocanis and "Candidatus Mycoplasma 
      haematoparvum," are commonly associated with immune-mediated haemolytic anaemia 
      (IMHA) in UK dogs. METHODS: Three groups of dogs were recruited to the study: 
      anaemic dogs with primary IMHA (n=37); anaemic dogs not meeting the inclusion 
      criteria for primary IMHA (n=77) and non-anaemic dogs (n=113). DNA was extracted 
      from 100 mul of blood and subjected to real-time quantitative polymerase chain 
      reaction (qPCR) assays for both species of Mycoplasma. Each assay incorporated 
      co-amplification of canine glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as an 
      endogenous internal control. RESULTS: Canine GAPDH was successfully amplified by 
      qPCR from all 227 canine blood samples but none contained M. haemocanis or 
      "Candidatus M. haematoparvum" DNA. CLINICAL SIGNIFICANCE: Haemoplasma infection 
      is uncommon in dogs in the UK and no evidence was found that these organisms act 
      as triggers for IMHA.
CI  - (c) 2010 British Small Animal Veterinary Association.
FAU - Warman, S M
AU  - Warman SM
AD  - School of Clinical Veterinary Science, University of Bristol, Langford House, 
      Langford, Bristol BS40 5DU.
FAU - Helps, C R
AU  - Helps CR
FAU - Barker, E N
AU  - Barker EN
FAU - Day, S
AU  - Day S
FAU - Sturgess, K
AU  - Sturgess K
FAU - Day, M J
AU  - Day MJ
FAU - Tasker, S
AU  - Tasker S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Small Anim Pract
JT  - The Journal of small animal practice
JID - 0165053
SB  - IM
MH  - Anemia, Hemolytic, Autoimmune/epidemiology/microbiology/*veterinary
MH  - Animals
MH  - Dog Diseases/*epidemiology/*microbiology
MH  - Dogs
MH  - Female
MH  - Male
MH  - Mycoplasma/pathogenicity
MH  - Mycoplasma Infections/complications/epidemiology/*veterinary
MH  - United Kingdom/epidemiology
EDAT- 2010/10/30 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/10/30 06:00
PHST- 2010/10/30 06:00 [entrez]
PHST- 2010/10/30 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1111/j.1748-5827.2010.00987.x [doi]
PST - ppublish
SO  - J Small Anim Pract. 2010 Oct;51(10):534-9. doi: 10.1111/j.1748-5827.2010.00987.x.