PMID- 21030715
OWN - NLM
STAT- MEDLINE
DCOM- 20110120
LR  - 20211020
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 31
IP  - 1
DP  - 2011 Jan
TI  - Macrophage adipose triglyceride lipase deficiency attenuates atherosclerotic 
      lesion development in low-density lipoprotein receptor knockout mice.
PG  - 67-73
LID - 10.1161/ATVBAHA.110.215814 [doi]
AB  - OBJECTIVE: The consequences of macrophage triglyceride (TG) accumulation on 
      atherosclerosis have not been studied in detail so far. Adipose triglyceride 
      lipase (ATGL) is the rate-limiting enzyme for the initial step in TG hydrolysis. 
      Because ATGL knockout (KO) mice exhibit massive TG accumulation in macrophages, 
      we used ATGL KO mice to study the effects of macrophage TG accumulation on 
      atherogenesis. METHODS AND RESULTS: Low-density lipoprotein receptor (LDLr) KO 
      mice were transplanted with bone marrow from ATGL KO (ATGL KO-->LDLr KO) or 
      wild-type (WT-->LDLr KO) mice and challenged with a Western-type diet for 9 weeks. 
      Despite TG accumulation in ATGL KO macrophages, atherosclerosis in ATGL KO-->LDLr 
      KO mice was 43% reduced associated with decreased plasma monocyte chemoattractant 
      protein-1 (MCP-1) and macrophage interleukin-6 concentrations. This coincided 
      with a reduced amount of macrophages, possibly because of a 39% increase in 
      intraplaque apoptosis and a decreased migratory capacity of ATGL KO macrophages. 
      The reduced number of white blood cells might be due to a 36% decreased 
      Lin(-)Sca-1(+)cKit(+) hematopoietic stem cell population. CONCLUSIONS: We 
      conclude that the attenuation of atherogenesis in ATGL KO-->LDLr KO mice is due to 
      decreased infiltration of less inflammatory macrophages into the arterial wall 
      and increased macrophage apoptosis.
FAU - Lammers, Bart
AU  - Lammers B
AD  - Division of Biopharmaceutics, Gorlaeus Laboratories, Einsteinweg 55, 2333CC 
      Leiden, the Netherlands. b.lammers@lacdr.leidenuniv.nl
FAU - Chandak, Prakash G
AU  - Chandak PG
FAU - Aflaki, Elma
AU  - Aflaki E
FAU - Van Puijvelde, Gijs H M
AU  - Van Puijvelde GH
FAU - Radovic, Branislav
AU  - Radovic B
FAU - Hildebrand, Reeni B
AU  - Hildebrand RB
FAU - Meurs, Illiana
AU  - Meurs I
FAU - Out, Ruud
AU  - Out R
FAU - Kuiper, Johan
AU  - Kuiper J
FAU - Van Berkel, Theo J C
AU  - Van Berkel TJ
FAU - Kolb, Dagmar
AU  - Kolb D
FAU - Haemmerle, Guenter
AU  - Haemmerle G
FAU - Zechner, Rudolf
AU  - Zechner R
FAU - Levak-Frank, Sanja
AU  - Levak-Frank S
FAU - Van Eck, Miranda
AU  - Van Eck M
FAU - Kratky, Dagmar
AU  - Kratky D
LA  - eng
GR  - F 3001/FWF_/Austrian Science Fund FWF/Austria
GR  - F 3002/FWF_/Austrian Science Fund FWF/Austria
GR  - F 3004/FWF_/Austrian Science Fund FWF/Austria
GR  - P 19186/FWF_/Austrian Science Fund FWF/Austria
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101028
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, LDL)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 3.1.1.3 (PNPLA2 protein, mouse)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Atherosclerosis/enzymology/etiology/genetics/immunology/pathology/*prevention & 
      control
MH  - Bone Marrow Transplantation
MH  - Carboxylic Ester Hydrolases/*deficiency/genetics
MH  - Cells, Cultured
MH  - Chemokine CCL2/blood
MH  - Chemotaxis
MH  - Cholesterol/blood
MH  - Diet, Atherogenic
MH  - Disease Models, Animal
MH  - Female
MH  - Gene Expression Regulation
MH  - Hematopoietic Stem Cells/metabolism
MH  - Hydrolysis
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-6/metabolism
MH  - Leukocyte Count
MH  - Lipase
MH  - Macrophages/*enzymology/immunology/pathology
MH  - Male
MH  - Mice
MH  - Mice, Knockout
MH  - Multipotent Stem Cells/metabolism
MH  - Receptors, LDL/*deficiency/genetics
MH  - Triglycerides/blood/*metabolism
MH  - Whole-Body Irradiation
PMC - PMC3063945
MID - UKMS33613
OID - NLM: UKMS33613
EDAT- 2010/10/30 06:00
MHDA- 2011/01/21 06:00
PMCR- 2011/03/25
CRDT- 2010/10/30 06:00
PHST- 2010/10/30 06:00 [entrez]
PHST- 2010/10/30 06:00 [pubmed]
PHST- 2011/01/21 06:00 [medline]
PHST- 2011/03/25 00:00 [pmc-release]
AID - ATVBAHA.110.215814 [pii]
AID - 10.1161/ATVBAHA.110.215814 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2011 Jan;31(1):67-73. doi: 
      10.1161/ATVBAHA.110.215814. Epub 2010 Oct 28.