PMID- 21030788
OWN - NLM
STAT- MEDLINE
DCOM- 20110308
LR  - 20190608
IS  - 1881-7122 (Electronic)
IS  - 0007-5124 (Linking)
VI  - 59
IP  - 5
DP  - 2010
TI  - Ovarian expression of inhibin-subunits, 3beta-hydroxysteroid dehydrogenase, and 
      cytochrome P450 aromatase during the estrous cycle and pregnancy of shiba goats 
      (Capra hircus).
PG  - 605-14
AB  - The cellular localization of the inhibin subunits (alpha, beta(A), and beta (B)), 
      steroidogenic enzymes (3beta-hydroxysteroid dehydrogenase (3betaHSD) and cytochrome 
      P450 aromatase (P450arom) were evaluated in the ovaries of cyclic (n=6) and 
      pregnant (n=2) Shiba goats (Capra Hircus). The immunointensity of inhibin alpha and 
      beta(A) subunits showed an increase in the granulosa cells (GC) of developing 
      follicles. Inhibin beta(B) subunit and P450arom showed high expression in GC of 
      antral follicles. 3betaHSD immunoreactivity was uniform in preantral and antral 
      follicles. In follicular phase and late pregnancy, there was a strong expression 
      of inhibin alpha subunit in GC of antral follicles. Although in mid pregnancy, antral 
      follicles GC showed moderate immunostaining of inhibin beta subunits, the 
      immunoreactivity of inhibin beta(A) and beta(B) subunits was high during the follicular 
      and luteal stages, respectively. While, immunoreactivity of GC to P450arom was 
      moderate during all studied stages, and 3betaHSD immunoreactivity was plentiful in 
      antral follicles during the luteal phase. The immunoreactivity to inhibin alpha 
      subunit and P450arom was abundant during mid pregnancy in the luteal tissues. 
      Immunoreaction to inhibin beta subunits was faint-to-moderate in cyclic and 
      pregnancy corpora lutea. Immunoexpression of 3betaHSD was maximal in late pregnancy 
      corpora lutea. The present results suggest that, in goats, the GC of antral 
      follicles are the main source of dimeric inhibins and that corpora lutea may 
      partially participate in the secretion of inhibin. Changes in ovarian hormonal 
      levels might depend on the synthesizing capacity of hormones in the follicles and 
      corpora lutea to regulate the goat's reproductive stages.
FAU - Kandiel, Mohamed M M
AU  - Kandiel MM
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty 
      of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
FAU - Watanabe, Gen
AU  - Watanabe G
FAU - Taya, Kazuyoshi
AU  - Taya K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 57285-09-3 (Inhibins)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/*metabolism
MH  - Animals
MH  - Aromatase/*metabolism
MH  - Estrous Cycle/*metabolism
MH  - Female
MH  - Goats/*physiology
MH  - Granulosa Cells/metabolism
MH  - Immunohistochemistry
MH  - Inhibins/*metabolism
MH  - Ovarian Follicle/metabolism
MH  - Ovary/*metabolism
MH  - Pregnancy
MH  - *Pregnancy, Animal
EDAT- 2010/10/30 06:00
MHDA- 2011/03/09 06:00
CRDT- 2010/10/30 06:00
PHST- 2010/10/30 06:00 [entrez]
PHST- 2010/10/30 06:00 [pubmed]
PHST- 2011/03/09 06:00 [medline]
AID - JST.JSTAGE/expanim/59.605 [pii]
AID - 10.1538/expanim.59.605 [doi]
PST - ppublish
SO  - Exp Anim. 2010;59(5):605-14. doi: 10.1538/expanim.59.605.