PMID- 21036160
OWN - NLM
STAT- MEDLINE
DCOM- 20110324
LR  - 20220310
IS  - 1873-3492 (Electronic)
IS  - 0009-8981 (Linking)
VI  - 412
IP  - 3-4
DP  - 2011 Jan 30
TI  - Lipid plasma concentrations of HDL subclasses determined by enzymatic staining on 
      polyacrylamide electrophoresis gels in children with metabolic syndrome.
PG  - 292-8
LID - 10.1016/j.cca.2010.10.021 [doi]
AB  - BACKGROUND: The antiatherogenic role of different HDL subclasses is still 
      controversial. HDL particles of the same size can have different lipid contents 
      in some physiopathological situations. However, little is known about the plasma 
      lipid levels of HDL subclasses when they are separated by their hydrodynamic 
      diameter. METHODS: Triglycerides (Tg), phosphatidylcholine (Ph), and cholesterol 
      (C) plasma concentrations of HDL subclasses, were determined by enzymatic 
      staining on polyacrylamide gradient gel (PAGE) in 50 pediatric patients with 
      metabolic syndrome (MS), and 50 control children paired by age and gender. 
      Proteins of HDL subclasses were also stained for the assessment of the relative 
      size distribution of HDL. RESULTS: Relative HDL size distribution was shifted to 
      small particles in MS pediatric patients when determined per protein. In 
      contrast, cholesterol plasma concentrations corresponding to the HDL2b, 2a, 3a, 
      and 3b subclasses were decreased; triglycerides of HDL3b and 3c, as well as 
      plasma phospholipids from HDL3c, were elevated in MS patients as compared to 
      controls. The C-to-Ph ratio, considered as indicative of HDL composition, was 
      similar among the 5 HDL subclasses in control subjects, whereas this ratio 
      gradually decreased from large HDL2b to small HDL3c in the MS group. Cholesterol 
      plasma concentrations of HDL subclasses correlated with the components of the MS. 
      CONCLUSIONS: Lipids of HDL subclasses provide more and accurate information than 
      the relative HDL size distribution determined by protein staining, and may 
      contribute to understand better HDL metabolism and the coronary risk associated 
      to these lipoproteins.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Garcia-Sanchez, Cynthia
AU  - Garcia-Sanchez C
AD  - Department of Molecular Biology, Instituto Nacional de Cardiologia Ignacio 
      Chavez, Juan Badiano 1, Seccion XVI, 14080 Mexico D.F., Mexico.
FAU - Torres-Tamayo, Margarita
AU  - Torres-Tamayo M
FAU - Juarez-Meavepena, Minerva
AU  - Juarez-Meavepena M
FAU - Lopez-Osorio, Cristhel
AU  - Lopez-Osorio C
FAU - Toledo-Ibelles, Paola
AU  - Toledo-Ibelles P
FAU - Monter-Garrido, Mariana
AU  - Monter-Garrido M
FAU - Cruz-Robles, David
AU  - Cruz-Robles D
FAU - Carreon-Torres, Elizabeth
AU  - Carreon-Torres E
FAU - Vargas-Alarcon, Gilberto
AU  - Vargas-Alarcon G
FAU - Perez-Mendez, Oscar
AU  - Perez-Mendez O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101028
PL  - Netherlands
TA  - Clin Chim Acta
JT  - Clinica chimica acta; international journal of clinical chemistry
JID - 1302422
RN  - 0 (Enzymes)
RN  - 0 (Lipoproteins, HDL)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Triglycerides)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Case-Control Studies
MH  - Child
MH  - Cholesterol/blood
MH  - Electrophoresis, Polyacrylamide Gel/*methods
MH  - Enzymes/*metabolism
MH  - Female
MH  - Humans
MH  - Lipoproteins, HDL/*blood/*isolation & purification
MH  - Male
MH  - Metabolic Syndrome/*blood
MH  - Phosphatidylcholines/blood
MH  - Surface Properties
MH  - Triglycerides/blood
EDAT- 2010/11/03 06:00
MHDA- 2011/03/25 06:00
CRDT- 2010/11/02 06:00
PHST- 2010/08/06 00:00 [received]
PHST- 2010/10/14 00:00 [revised]
PHST- 2010/10/22 00:00 [accepted]
PHST- 2010/11/02 06:00 [entrez]
PHST- 2010/11/03 06:00 [pubmed]
PHST- 2011/03/25 06:00 [medline]
AID - S0009-8981(10)00652-2 [pii]
AID - 10.1016/j.cca.2010.10.021 [doi]
PST - ppublish
SO  - Clin Chim Acta. 2011 Jan 30;412(3-4):292-8. doi: 10.1016/j.cca.2010.10.021. Epub 
      2010 Oct 28.