PMID- 21036383
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20131121
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 82
IP  - 3
DP  - 2011 Jan
TI  - Transcriptome analysis provides insights for understanding the adverse effects of 
      endosulfan in Drosophila melanogaster.
PG  - 370-6
LID - 10.1016/j.chemosphere.2010.10.002 [doi]
AB  - Indiscriminate use of agrochemicals worldwide, particularly, persistent organic 
      pollutants (POPs), is of concern. Endosulfan, a POP, is used by various 
      developing/developed nations and is known to adversely affect the development and 
      the hormonal profiles of humans and animals. However, little is known about the 
      molecular players/pathways underlying the adverse effects of endosulfan. We 
      therefore analyzed the global gene expression changes and subsequent adverse 
      effects of endosulfan using Drosophila. We used Drosophila melanogaster keeping 
      in view of its well annotated genome and the wealth of genetic/molecular reagents 
      available for this model organism. We exposed third instar larvae of D. 
      melanogaster to endosulfan (2.0 mug mL(-1)) for 24 h and using microarray, we 
      identified differential expression of 256 genes in exposed organisms compared to 
      controls. These genes are associated with cellular processes such as development, 
      stress and immune response and metabolism. Microarray results were validated 
      through quantitative PCR and biochemical assay on a subset of genes/proteins. 
      Taking cues from microarray data, we analyzed the effect of endosulfan on 
      development, emergence and survival of the organism. In exposed organisms, we 
      observed deformities in hind-legs, reminiscent of those observed in higher 
      organisms exposed to endosulfan. In addition, we observed delayed and/or reduced 
      emergence in exposed organisms when compared to their respective controls. 
      Together, our studies not only highlight the adverse effects of endosulfan on the 
      organism but also provide an insight into the possible genetic perturbations 
      underlying these effects, which might have potential implications to higher 
      organisms.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Sharma, Anurag
AU  - Sharma A
AD  - Embryotoxicology Section, Indian Institute of Toxicology Research, Lucknow 226 
      001, India.
FAU - Mishra, M
AU  - Mishra M
FAU - Ram, K Ravi
AU  - Ram KR
FAU - Kumar, R
AU  - Kumar R
FAU - Abdin, M Z
AU  - Abdin MZ
FAU - Chowdhuri, D Kar
AU  - Chowdhuri DK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101030
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Environmental Pollutants)
RN  - 0 (Insecticides)
RN  - 1398-61-4 (Chitin)
RN  - OKA6A6ZD4K (Endosulfan)
SB  - IM
MH  - Abnormalities, Drug-Induced
MH  - Animals
MH  - Chitin/metabolism
MH  - Drosophila melanogaster/*drug effects/genetics/metabolism
MH  - Endosulfan/*toxicity
MH  - Environmental Pollutants/toxicity
MH  - Gene Expression/*drug effects
MH  - Gene Expression Profiling
MH  - Genes, Insect
MH  - Insecticides/*toxicity
MH  - Larva/drug effects/genetics/metabolism
EDAT- 2010/11/03 06:00
MHDA- 2011/01/25 06:00
CRDT- 2010/11/02 06:00
PHST- 2010/04/05 00:00 [received]
PHST- 2010/09/24 00:00 [revised]
PHST- 2010/10/03 00:00 [accepted]
PHST- 2010/11/02 06:00 [entrez]
PHST- 2010/11/03 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
AID - S0045-6535(10)01134-3 [pii]
AID - 10.1016/j.chemosphere.2010.10.002 [doi]
PST - ppublish
SO  - Chemosphere. 2011 Jan;82(3):370-6. doi: 10.1016/j.chemosphere.2010.10.002. Epub 
      2010 Oct 30.