PMID- 21041979
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20191111
IS  - 1880-5779 (Electronic)
IS  - 1341-7568 (Linking)
VI  - 85
IP  - 3
DP  - 2010
TI  - Molecular characterization of Myostatin gene from Zhikong scallop Chlamys farreri 
      (Jones et Preston 1904).
PG  - 207-18
AB  - The scallop is an economically important sea food prized for its large and 
      delicious adductor muscle. Studying the molecular basis of scallop muscle growth 
      is important for both scallop breeding and our understanding of muscle mass 
      regulation in bivalve. Myostatin (MSTN) is a conserved negative regulator of 
      muscle growth and development. Here we report the MSTN gene from Zhikong scallop 
      (Chlamys farreri Jones et Preston 1904). The C. farreri MSTN consists of 11651 
      nucleotides encoding 457 amino acids. The gene has a 3-exon/2-intron structure 
      that is conserved with vertebrate homologs. The exons are 586, 380 and 408 bp in 
      length, respectively, and separated by introns of 5086 and 1518 bp. The protein 
      sequence contains characteristic conserved residues including a cleavage motif of 
      proteolysis (RXXR) and nine cysteines. Three transcription initiation sites were 
      found at 62, 146, and 296 bp upstream of the translation start codon ATG. In 
      silico analysis of the promoter region identified a TATA-box and several 
      muscle-specific regulatory elements including COMP, MEF2s, MTBFs and E-boxes. 
      Minisatellite DNA was found in intron 1. By fluorescence in situ hybridization 
      (FISH), the gene was mapped to the long arm of a pair of middle subtelocentric 
      chromosome. Quantitative analysis of MSTN transcripts in embryos/larvae indicated 
      high expression level in gastrulae and limited expression at other stages. In 
      adult scallops, MSTN is predominantly expressed in striated muscle, with 
      different expression levels in other tissues. Our data provide valuable genomic 
      and expression information which will aid the further study on scallop MSTN 
      function and MSTN evolution.
FAU - Hu, Xiaoli
AU  - Hu X
AD  - Key Laboratory of Marine Genetics and Breeding (Ocean University of China), 
      Ministry of Education, Qingdao, China.
FAU - Guo, Huihui
AU  - Guo H
FAU - He, Yan
AU  - He Y
FAU - Wang, Shan
AU  - Wang S
FAU - Zhang, Lingling
AU  - Zhang L
FAU - Wang, Shi
AU  - Wang S
FAU - Huang, Xiaoting
AU  - Huang X
FAU - Roy, Scott William
AU  - Roy SW
FAU - Lu, Wei
AU  - Lu W
FAU - Hu, Jingjie
AU  - Hu J
FAU - Bao, Zhenmin
AU  - Bao Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Genes Genet Syst
JT  - Genes & genetic systems
JID - 9607822
RN  - 0 (Myostatin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chromosomes
MH  - Cloning, Molecular
MH  - *Evolution, Molecular
MH  - Exons
MH  - Gene Expression Profiling
MH  - Genome
MH  - Introns
MH  - Minisatellite Repeats
MH  - Molecular Sequence Data
MH  - Myostatin/*chemistry/genetics
MH  - Pectinidae/*genetics
MH  - *Phylogeny
MH  - Sequence Alignment
EDAT- 2010/11/03 06:00
MHDA- 2011/02/11 06:00
CRDT- 2010/11/03 06:00
PHST- 2010/11/03 06:00 [entrez]
PHST- 2010/11/03 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
AID - JST.JSTAGE/ggs/85.207 [pii]
AID - 10.1266/ggs.85.207 [doi]
PST - ppublish
SO  - Genes Genet Syst. 2010;85(3):207-18. doi: 10.1266/ggs.85.207.