PMID- 21042905
OWN - NLM
STAT- MEDLINE
DCOM- 20110523
LR  - 20181201
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 399
IP  - 3
DP  - 2011 Jan
TI  - Acridine orange-induced signal enhancement effect of tyrosinase-immobilized 
      carbon-felt-based flow biosensor for highly sensitive detection of monophenolic 
      compounds.
PG  - 1151-62
LID - 10.1007/s00216-010-4369-1 [doi]
AB  - Tyrosinase (TYR: EC 1.14.18.1) was covalently modified onto the surface of a 
      cyanuric chloride-activated carbon felt (CF) from the mixed buffer solution of 
      TYR and acridine orange (AO). The resulting TYR-immobilized CF (TYR/AO-CF) was 
      used as a working electrode unit of an electrochemical flow-through detector for 
      mono- and di-phenolic compounds (i.e., p-chlorophenol (p-CP), p-cresol, phenol, 
      and catechol), which detects the reduction current of enzymatically produced 
      o-quinones at -0.05 V (vs. Ag/AgCl). The presence of AO (0.2 mM) in TYR solution 
      during the enzyme immobilization step was significantly effective for the signal 
      enhancements especially for p-CP, and the cathodic peak currents of p-CP by the 
      TYR/AO-CF-based detector were much larger than those by the TYR-CF-based detector 
      prepared from TYR solution without AO. The oxymetry with Clark-type oxygen 
      electrode revealed that monophenolase activity of free TYR in 1 mM phosphate 
      buffer (pH 7.0) was greatly enhanced in the presence of AO (0.2 mM), whereas 
      diphenolase activity was not so much influenced. Furthermore, the comparison of 
      cyclic voltammograms of TYR/AO-CF and TYR-CF in air-saturated phosphate buffer 
      containing each substrate revealed that the electrochemical reduction rate of 
      p-chloro-o-benzoquinone at TYR/AO-CF was faster than that at TYR-CF. In addition, 
      the electrochemical impedance spectroscopy revealed that the structural 
      properties of immobilized TYR on the CF would be influenced by AO. Some kinds of 
      interaction of AO with TYR would affect the enzymatic kinetics and the structural 
      properties of the immobilized TYR, leading to the signal enhancement of the 
      TYR-CF-based flow biosensor especially for monophenolic compounds.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Materials Science and Engineering, Graduate School of Engineering, 
      Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama 369-0293, Japan.
FAU - Hasebe, Yasushi
AU  - Hasebe Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101103
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Carbon Fiber)
RN  - 0 (Catechols)
RN  - 0 (Chlorophenols)
RN  - 0 (Cresols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Triazines)
RN  - 1MXY2UM8NV (4-cresol)
RN  - 3DLC36A01X (4-chlorophenol)
RN  - 5U4L4QHD6I (cyanuric chloride)
RN  - 7440-44-0 (Carbon)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - F30N4O6XVV (Acridine Orange)
RN  - LF3AJ089DQ (catechol)
SB  - IM
MH  - Acridine Orange/*pharmacology
MH  - *Biosensing Techniques
MH  - Carbon/*chemistry
MH  - Carbon Fiber
MH  - Catechols/analysis
MH  - Chlorophenols/analysis
MH  - Cresols/analysis
MH  - Enzymes, Immobilized/chemistry/*metabolism
MH  - Monophenol Monooxygenase/chemistry/*metabolism
MH  - Sensitivity and Specificity
MH  - Surface Properties
MH  - Triazines/chemistry
EDAT- 2010/11/03 06:00
MHDA- 2011/05/24 06:00
CRDT- 2010/11/03 06:00
PHST- 2010/09/11 00:00 [received]
PHST- 2010/10/19 00:00 [accepted]
PHST- 2010/10/16 00:00 [revised]
PHST- 2010/11/03 06:00 [entrez]
PHST- 2010/11/03 06:00 [pubmed]
PHST- 2011/05/24 06:00 [medline]
AID - 10.1007/s00216-010-4369-1 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2011 Jan;399(3):1151-62. doi: 10.1007/s00216-010-4369-1. Epub 
      2010 Nov 3.