PMID- 21044631
OWN - NLM
STAT- MEDLINE
DCOM- 20110617
LR  - 20131121
IS  - 1879-0135 (Electronic)
IS  - 0020-7519 (Linking)
VI  - 41
IP  - 3-4
DP  - 2011 Mar
TI  - Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in 
      P2X7 receptor-deficient mice.
PG  - 301-8
LID - 10.1016/j.ijpara.2010.10.001 [doi]
AB  - The P2X(7) receptor (P2X(7)R) is a two transmembrane receptor that is highly 
      expressed on the surface of immune cells. Loss of function polymorphisms in this 
      receptor have been linked to increased susceptibility to intracellular pathogens. 
      P2X(7)R gene knockout (P2X(7)R(-/-); on a C57Bl/6J background), C57Bl/6J and 
      BALB/c mice were infected with the avirulent ME49 strain of the intracellular 
      parasite, Toxoplasma gondii, and susceptibility determined by monitoring weight 
      loss. P2X(7)R(-/-) mice lost significantly more weight than C57Bl/6J mice from 
      day 8p.i. C57Bl/6J, in turn, lost significantly more weight than BALB/c mice. 
      Thus, by day 10p.i., P2X(7)R(-/-) mice had lost 5.7 +/- 0.7% of their weight versus 
      2.4 +/- 0.6% for C57Bl/6J mice, whereas BALB/c mice had gained 1.9 +/- 0.5%; by day 
      12p.i., P2X(7)R(-/-) mice had lost 15.1+/-0.6%, C57Bl/6J had lost 10.1+/-0.8% and 
      BALB/c had lost 4.8 +/- 0.8% of their weight. Neither parasite burden nor liver 
      pathology was greater in the P2X(7)R(-/-) mice than in C57Bl/6J mice but BALB/c 
      mice had significantly smaller numbers of parasites and less pathology in their 
      livers than these strains. Absence of the P2X(7) receptor did not affect IFN-gamma, 
      IL-12, IL-1beta, monocyte chemoattractant protein-1 (MCP-1) or TNF production. 
      However, both P2X(7)R(-/-) and C57Bl/6J mice produced more IL-1beta and TNF than 
      BALB/c mice. There was one important point of differentiation between the 
      P2X(7)R(-/-) and C57Bl/6J mice, namely the significantly enhanced and prolonged 
      production of nitric oxide, accompanied by delayed production of IL-10 in the 
      P2X(7)R-deficient mice.
CI  - Copyright (c) 2010 Australian Society for Parasitology Inc. Published by Elsevier 
      Ltd. All rights reserved.
FAU - Miller, Catherine M
AU  - Miller CM
AD  - Institute for Biotechnology of Infectious Diseases, University of Technology, 
      Sydney, Broadway, NSW 2007, Australia.
FAU - Zakrzewski, Alana M
AU  - Zakrzewski AM
FAU - Ikin, Rowan J
AU  - Ikin RJ
FAU - Boulter, Nicola R
AU  - Boulter NR
FAU - Katrib, Marilyn
AU  - Katrib M
FAU - Lees, Michael P
AU  - Lees MP
FAU - Fuller, Stephen J
AU  - Fuller SJ
FAU - Wiley, James S
AU  - Wiley JS
FAU - Smith, Nicholas C
AU  - Smith NC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101031
PL  - England
TA  - Int J Parasitol
JT  - International journal for parasitology
JID - 0314024
RN  - 0 (Receptors, Purinergic P2X7)
RN  - 130068-27-8 (Interleukin-10)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Disease Susceptibility
MH  - Inflammation/*immunology/*parasitology/physiopathology
MH  - Interleukin-10/biosynthesis
MH  - Liver/parasitology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Nitric Oxide/biosynthesis
MH  - Receptors, Purinergic P2X7/*deficiency/genetics
MH  - Species Specificity
MH  - Toxoplasma/immunology/*pathogenicity
MH  - Toxoplasmosis, Animal/genetics/*immunology/parasitology/*physiopathology
MH  - Weight Loss
EDAT- 2010/11/04 06:00
MHDA- 2011/06/18 06:00
CRDT- 2010/11/04 06:00
PHST- 2010/09/26 00:00 [received]
PHST- 2010/10/04 00:00 [accepted]
PHST- 2010/11/04 06:00 [entrez]
PHST- 2010/11/04 06:00 [pubmed]
PHST- 2011/06/18 06:00 [medline]
AID - S0020-7519(10)00324-3 [pii]
AID - 10.1016/j.ijpara.2010.10.001 [doi]
PST - ppublish
SO  - Int J Parasitol. 2011 Mar;41(3-4):301-8. doi: 10.1016/j.ijpara.2010.10.001. Epub 
      2010 Oct 31.