PMID- 21046787
OWN - NLM
STAT- MEDLINE
DCOM- 20120213
LR  - 20221207
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 28
IP  - 7
DP  - 2010 Jul
TI  - [Simultaneous determination of berberine, palmatine, matrine, catechin and 
      baicalin in Funing Shuan by micellar electrokinetic capillary 
      chromatography-electrospray ionization mass spectrometry].
PG  - 677-81
AB  - A method for the simultaneous determination of berberine, palmatine, matrine, 
      catechin and baicalin in Funing Shuan was established using micellar 
      electrokinetic capillary chromatography-electrospray ionization mass spectrometry 
      (MEKC-ESI MS). The compounds were separated on an uncoated capillary (80 cm x 50 
      microm) with the operating voltage of 25 kV and the running buffer of 40 mmol/L 
      lauric acid-100 mmol/L ammonia mixture containing 25% acetonitrile (pH 9.5). The 
      baseline separation of the five compounds was achieved within 16 min with a 
      satisfactory repeatability and sensitivity. The solution of 50% 2-propanol/water 
      solution (containing 3 mmol/L acetic acid) was used as the sheath liquid for the 
      ESI MS analysis. The results showed that the linear ranges for berberine, 
      palmatine, matrine, catechin and baicalin were 0.03 - 15, 0.05 - 15, 0.2 - 250, 
      1.5 - 300 and 2.0 - 500 mg/L, respectively, and the detection limits were 0.01, 
      0.02, 0.05, 0.5 and 0.6 mg/L, respectively. The average recoveries of the five 
      components were between 94.0% - 104.0% with the relative standard deviations 
      (RSDs) of 0.3% - 3.2%. The developed method is simple, rapid, and accurate, and 
      it is suitable for the routine analysis of the five effective components in 
      Funing Shuan.
FAU - Zeng, Yongfang
AU  - Zeng Y
AD  - Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, 
      Nanning 530004, China.
FAU - Huo, Peng
AU  - Huo P
FAU - Xu, Yuanjin
AU  - Xu Y
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Alkaloids)
RN  - 0 (Berberine Alkaloids)
RN  - 0 (Drug Combinations)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Quinolizines)
RN  - 0I8Y3P32UF (Berberine)
RN  - 347Q89U4M5 (baicalin)
RN  - 8R1V1STN48 (Catechin)
RN  - G50C034217 (palmatine)
RN  - 0 (Matrines)
SB  - IM
MH  - Alkaloids/analysis
MH  - Berberine/*analysis
MH  - Berberine Alkaloids/*analysis
MH  - Capillary Electrochromatography/*methods
MH  - Catechin/analysis
MH  - Drug Combinations
MH  - Drugs, Chinese Herbal/*analysis
MH  - Flavonoids/analysis
MH  - Humans
MH  - Quinolizines/analysis
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
MH  - Matrines
EDAT- 2010/11/05 06:00
MHDA- 2012/02/14 06:00
CRDT- 2010/11/05 06:00
PHST- 2010/11/05 06:00 [entrez]
PHST- 2010/11/05 06:00 [pubmed]
PHST- 2012/02/14 06:00 [medline]
AID - 10.3724/sp.j.1123.2010.00677 [doi]
PST - ppublish
SO  - Se Pu. 2010 Jul;28(7):677-81. doi: 10.3724/sp.j.1123.2010.00677.